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  • KD/KO Validated

MARS Polyclonal antibody

MARS Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14829-1-AP

Synonyms

MARS, MARS1, Methionine tRNA ligase, methionyl tRNA synthetase, METRS, MTRNS



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

14829-1-AP targets MARS in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MARS fusion protein Ag6619
Full Name methionyl-tRNA synthetase
Calculated molecular weight 101 kDa
Observed molecular weight 101 kDa
GenBank accession numberBC002384
Gene symbol MARS
Gene ID (NCBI) 4141
RRIDAB_2140917
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MARS antibody 14829-1-APDownload protocol
IHC protocol for MARS antibody 14829-1-APDownload protocol
IF protocol for MARS antibody 14829-1-APDownload protocol
IP protocol for MARS antibody 14829-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Proteomics

Motile hepatocellular carcinoma cells preferentially secret sugar metabolism regulatory proteins via exosomes.

Authors - Jing Zhang
humanWB,IHC

J Clin Pathol

High methionyl-tRNA synthetase expression predicts poor prognosis in patients with breast cancer.

Authors - Qin Jin
  • KD Validated
humanWB

Proc Natl Acad Sci U S A

Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex

Authors - Krishnendu Khan
  • KD Validated
mouseWB

Aging Cell

N-homocysteinylation of α-synuclein promotes its aggregation and neurotoxicity

Authors - Lingyan Zhou
  • KD Validated