MAST2 Polyclonal antibody

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• MAST2 Polyclonal antibody (33335-1-AP)

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  WB analysis using 33335-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 33335-1-AP (MAST2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 33335-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using MAST2 antibody (33335-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of U2OS using 33335-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using MAST2 antibody (33335-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

MAST2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 33335-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

EC:2.7.11.1, KIAA0807, MAST205, Microtubule-associated serine/threonine-protein kinase 2

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, Jurkat cells
• Positive IF/ICC detected in: U2OS cells, A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33335-1-AP targets MAST2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35818

  Product name: Recombinant human MAST2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1480-1798 aa of NM_015112.2

  Sequence: TLRQDRAERRESLQKQEAIREVDSSEDDTEEGPENSQGAQELSLAPHPEVSQSVAPKGAGESGEEDPFPSRDPRSLGPMVPSLLTGITLGPPRMESPSGPHRRLGSPQAIEEAASSSSAGPNLGQSGATDPIPPEGCWKAQHLHTQALTALSPSTSGLTPTSSCSPPSSTSGKLSMWSWKSLIEGPDRASPSRKATMAGGLANLQDLENTTPAQPKNLSPREQGKTQPPSAPRLAHPSYEDPSQGWLWESECAQAVKEDPALSITQVPDASGDRRQDVPCRGCPLTQKSEPSLRRGQEPGGHQKHRDLALVPDELLKQT

• Full Name: microtubule associated serine/threonine kinase 2
• Calculated Molecular Weight: 196kDa，1798aa
• Observed Molecular Weight: 196 kDa
• GenBank Accession Number: NM_015112.2
• Gene Symbol: MAST2
• Gene ID (NCBI): 23139
• RRID: AB_3742944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q6P0Q8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAST2 is a microtubule-associated serine/threonine kinase, belonging to an evolutionarily conserved branch of AGC protein kinase superfamily. Since its discovery in 1993, it has been widely expressed in many tissues, including brain, heart, lung, liver, intestine and kidney. MAST2 is associated with many diseases, such as breast cancer, liver cancer, myeloma, inflammatory bowel disease, cystic fibrosis and various neurological diseases.