MASTL Polyclonal antibody

MASTL Polyclonal Antibody for IHC, ELISA

Cat No. 15739-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, ELISA

Synonyms

GWL, hGWL, MASTL, MAST-L, RP11 85G18.2, THC2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllIHC

IHC staining of human spleen using 15739-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15739-1-AP (MASTL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human spleen using 15739-1-AP
Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 15739-1-AP (MASTL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 15739-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15739-1-AP (MASTL antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human tonsillitis using 15739-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15739-1-AP (MASTL antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse spleen using 15739-1-AP
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15739-1-AP (MASTL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive IHC detected in

human spleen tissue, mouse spleen tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Application	Dilution
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15739-1-AP targets MASTL in IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9397 Product name: Recombinant human MASTL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 34-219 aa of BC009107 Sequence: EFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQVLSLISSLG Predict reactive species
Full Name	microtubule associated serine/threonine kinase-like
Calculated Molecular Weight	879 aa, 97 kDa
GenBank Accession Number	BC009107
Gene Symbol	MASTL
Gene ID (NCBI)	84930
RRID	AB_2878178
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96GX5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for MASTL antibody 15739-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	IHC	Oncol Lett Mastl overexpression is associated with epithelial to mesenchymal transition and predicts a poor clinical outcome in gastric cancer. Authors - Xian-Jun Sun View Article