Tested Applications
| Positive IHC detected in | human spleen tissue, mouse spleen tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15739-1-AP targets MASTL in IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9397 Product name: Recombinant human MASTL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 34-219 aa of BC009107 Sequence: EFSIVKPISRGAFGKVYLGQKGGKLYAVKVVKKADMINKNMTHQVQAERDALALSKSPFIVHLYYSLQSANNVYLVMEYLIGGDVKSLLHIYGYFDEEMAVKYISEVALALDYLHRHGIIHRDLKPDNMLISNEGHIKLTDFGLSKVTLNRDINMMDILTTPSMAKPRQDYSRTPGQVLSLISSLG Predict reactive species |
| Full Name | microtubule associated serine/threonine kinase-like |
| Calculated Molecular Weight | 879 aa, 97 kDa |
| GenBank Accession Number | BC009107 |
| Gene Symbol | MASTL |
| Gene ID (NCBI) | 84930 |
| RRID | AB_2878178 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96GX5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MASTL antibody 15739-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MASTL Polyclonal antibody (Cat# 15739-1-AP) has 1 citation. It was used in IHC in a study published in Oncology Letters (2017), investigating MASTL overexpression in relation to epithelial-to-mesenchymal transition (EMT) and its association with poor clinical outcomes in gastric cancer. The research falls within the fields of oncology, cancer biology, and cell biology.
| Species | Application | Title |
|---|---|---|
Oncol Lett Mastl overexpression is associated with epithelial to mesenchymal transition and predicts a poor clinical outcome in gastric cancer. |
Reviews
What customers say
The single review for this antibody is highly positive, with a 5-star rating. The reviewer used it for Western Blot at a 1:500 dilution on a renal cancer cell line and reported that it worked great. Overall, the feedback indicates strong performance and reliability for WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Balawant (Verified Customer) (08-24-2022) | I have used this Antibody. it is working great.
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