|Positive WB detected in||mouse liver tissue, arabidopsis whole plant|
|Positive IHC detected in||human lung tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:400|
|Sample-dependent, check data in validation data gallery|
12395-1-AP targets MAT1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, arabidopsis samples.
|Tested Reactivity||human, mouse, rat, arabidopsis|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||MAT1A fusion protein Ag3076|
|Full Name||methionine adenosyltransferase I, alpha|
|Calculated molecular weight||395 aa, 44 kDa|
|Observed molecular weight||44-55 kDa|
|GenBank accession number||BC018359|
|Gene ID (NCBI)||4143|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.1% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and may cross-react with MAT2A. Western blot analysis using this antibody detected a single band around 44-50 kDa in liver.
Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice.
Ann N Y Acad Sci
J Surg Oncol
Histological expression of methionine adenosyl transferase (MAT) 2A as a post-surgical prognostic surrogate in patients with hepatocellular carcinoma.
Mech Ageing Dev
Sulfur-based redox alterations in long-lived Snell dwarf mice.
Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.
Metabolomic Analysis Reveals Metabolic Changes Caused By Bisphenol A in Rats.