MAT1A Polyclonal antibody

MAT1A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, arabidopsis

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12395-1-AP

Synonyms

AdoMet synthase 1, AMS1, MAT, MAT 1, MAT I/III, MAT1A, MATA1, SAMS, SAMS1



Tested Applications

Positive WB detected inmouse liver tissue, arabidopsis whole plant
Positive IHC detected inhuman lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:400
Sample-dependent, check data in validation data gallery

Product Information

12395-1-AP targets MAT1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, arabidopsis samples.

Tested Reactivity human, mouse, rat, arabidopsis
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAT1A fusion protein Ag3076
Full Name methionine adenosyltransferase I, alpha
Calculated molecular weight 395 aa, 44 kDa
Observed molecular weight 44-55 kDa
GenBank accession numberBC018359
Gene symbol MAT1A
Gene ID (NCBI) 4143
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Methionine adenosyltransferase (MAT) is the enzyme that catalyzes the synthesis of S-adeno-sylmethionine (AdoMet), the main donor of methyl groups in the cell. In mammals MAT is encoded by two different genes, MAT1A and MAT2A. MAT1A is expressed only in the mature liver whereas fetal hepatocytes, extrahepatic tissues and liver cancer cells express MAT2A. This antibody was raised against the full length of human MAT1A and may cross-react with MAT2A. Western blot analysis using this antibody detected a single band around 44-50 kDa in liver.

Protocols

Product Specific Protocols
WB protocol for MAT1A antibody 12395-1-APDownload protocol
IHC protocol for MAT1A antibody 12395-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Longev Healthspan

Altered dietary methionine differentially impacts glutathione and methionine metabolism in long-living growth hormone-deficient Ames dwarf and wild-type mice.

Authors - Holly M Brown-Borg
human

Ann N Y Acad Sci

PMID: 29722030

Authors - Holly M Brown-Borg
humanIHC

J Surg Oncol

Histological expression of methionine adenosyl transferase (MAT) 2A as a post-surgical prognostic surrogate in patients with hepatocellular carcinoma.

Authors - Jihyun An
mouseWB

Mech Ageing Dev

Sulfur-based redox alterations in long-lived Snell dwarf mice.

Authors - Vitvitsky Victor V
mouseWB

Proteomics

Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.

Authors - Thomas Anja A
ratWB

Toxicol Sci

Metabolomic Analysis Reveals Metabolic Changes Caused By Bisphenol A in Rats.

Authors - Minjian Chen