Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells |
| Positive IHC detected in | human lung cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12202-2-AP targets MATR3 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2843 Product name: Recombinant human MATR3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 495-847 aa of BC015031 Sequence: GRVIHLSNLPHSGYSDSAVLKLAEPYGKIKNYILMRMKSQAFIEMETREDAMAMVDHCLKKALWFQGRCVKVDLSEKYKKLVLRIPNRGIDLLKKDKSRKRSYSPDGKESPSDKKSKTDGSQKTESSTEGKEQEEKSGEDGEKDTKDDQTEQEPNMLLESEDELLVDEEEAAALLESGSSVGDETDLANLGDVASDGKKEPSDKAVKKDGSASAAAKKKLKKVDKIEELDQENEAALENGIKNEENTEPGAESSENADDPNKDTSENADGQSDENKDDYTIPDEYRIGPYQPNVPVGIDYVIPKTGFYCKLCSLFYTNEEVAKNTHCSSLPHYQKLKKFLNKLAEERRQKKET Predict reactive species |
| Full Name | matrin 3 |
| Calculated Molecular Weight | 847 aa, 95 kDa |
| Observed Molecular Weight | 125 kDa |
| GenBank Accession Number | BC015031 |
| Gene Symbol | MATR3 |
| Gene ID (NCBI) | 9782 |
| RRID | AB_2281752 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43243 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MATR3, known as KIAA0723, is an 847 amino acid protein, which may play a role in transcription or may interact with other nuclear matrix proteins to form the internal fibrogranular network. MATR3 was originally identified as a nuclear matrix protein containing both DNA and RNA binding domains (PMID: 2033075) with a wide variety of functions ranging from DNA damage response to transcriptional and post-transcriptional regulation of cellular and viral RNAs(PMID: 21771346). the calculated molecular weight of MATR3 is 94 kDa, but we detect a 125 kDa protein as a result of PMID: 28977530.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MATR3 antibody 12202-2-AP | Download protocol |
| IHC protocol for MATR3 antibody 12202-2-AP | Download protocol |
| WB protocol for MATR3 antibody 12202-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MATR3 antibody (catalog no. 12202-2-AP) has 20 citations. These appear in journals including Cell, Nature Communications, Neuron, Brain, Cell Reports, Cell Death & Disease, Journal of Cell Biology, Journal of Biological Chemistry, RNA Biology, Analytical Chemistry, Breast Cancer Research, Journal of Ovarian Research, iScience, Clinical Immunology, and bioRxiv. Research fields span RNA biology and stress granules, neurodegeneration (ALS, Huntington's disease), epigenetics and m6A modification, paraspeckle biology, and cancer.
| Species | Application | Title |
|---|---|---|
Nat Commun TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV. | ||
Nat Commun An oncopeptide regulates m6A recognition by the m6A reader IGF2BP1 and tumorigenesis. | ||
Neuron Targeting PGAM5-driven mitochondrial integrated stress response slows ALS progression across subtypes. | ||
Brain STMN2 protein depletion via translation deficits and stress granules in amyotrophic lateral sclerosis. |
Reviews
What customers say
All three reviews rate the product 5 stars. Two Western Blot users report a strong, specific ~100 kDa MATR3 band at a 1:1000 dilution in HeLa, Jurkat, and HEK293 cells, with one noting a 1-hour room-temperature incubation was sufficient. An immunofluorescence user observed good, specific nuclear staining in SH-SY5Y cells at 1:1000 using 4% PFA fixation and methanol permeabilization. Overall, reviewers highlight reliable specificity, consistent performance across cell lines, and ease of use at a standard 1:1000 dilution.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Roy (Verified Customer) (06-12-2024) | Strong MATR3 band detected with a 1/1000 dilution in HeLa and Jurkat cells (1h incubation at RT)
|
Tatyana (Verified Customer) (03-11-2023) | Good and specific nuclear staining. 4% PFA fixation followed by methanol permeabilization; antibody prepared in 5% goat serum in PBST; 2 h at RT incubation.
 |
Wael (Verified Customer) (08-22-2022) | around 50ug of total cell lysate Blocking: 5% skim milk in PBS Primary incubation: 1:1000 in 5% skim milk in PBS overnight, rotating 4C Washes : 3X 15mins of PBS(0.001% Tween) Secondary: 1:10000 in 5% skim milk in PBS, 1hr, RT Washes : 3X 15mins of PBS(0.001% Tween) Specific Band size around 100KD
 |
