MAX Recombinant monoclonal antibody

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  WB analysis using 82958-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82958-6-RR (MAX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 82958-6-RR

  Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using MAX antibody (82958-6-RR, Clone: 230215D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 82958-6-RR

  Biolayer interferometry (BLl) kinetic assays of 82958-6-RR against Human MAX were performed. The affinity constant is 0.257 nM.

MAX Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 82958-6-RR

Clone No.230215D9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

bHLHd4, bHLHd5, bHLHd6, bHLHd7, bHLHd8

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Tested Applications

• Positive WB detected in: PC-3 cells, HEK-293 cells, HL-60 cells, A431 cells, Ramos cells, NIH/3T3 cells, C6 cells
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82958-6-RR targets MAX in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag0680

  Product name: Recombinant human MAX protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-151 aa of BC003525

  Sequence: MSDNDDIEVESDADKRAHHNALERKRRDHIKDSFHSLRDSVPSLQGEKASRAQILDKATEYIQYMRRKNHTHQQDIDDLKRQNALLEQQVRALEKARSSAQLQTNYPSSDNSLYTNAKGSTISAFDGGSDSSSESEPEEPQSRKKLRMEAS

• Full Name: MYC associated factor X
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC003525
• Gene Symbol: MAX
• Gene ID (NCBI): 4149
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61244
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Max, a member of MAX family, contains a basic helix-loop-helix (bHLH) domain, is a transcription regulator. MAX can form a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, while the MAD-MAX complex is a repressor. MAX could dimerizated with another bHLH protein to form a heterodimer, such MYC or MAD. Thus bands recognized by this antibody much larger than predicted.