MAZ Polyclonal antibody

MAZ Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 21068-1-AP

Synonyms

Purine-binding transcription factor, Pur-1, PUR1, Pur 1, Myc-associated zinc finger protein

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Validation Data Gallery

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  WB analysis of HeLa using 21068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21068-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21068-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21068-1-AP (MAZ antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of A431 using 21068-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using MAZ antibody (21068-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse lung tissue
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

21068-1-AP targets MAZ in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAZ fusion protein Ag15200
• Full Name: MYC-associated zinc finger protein (purine-binding transcription factor)
• Calculated Molecular Weight: 477 aa, 49 kDa
• Observed Molecular Weight: 49-55 kDa
• GenBank Accession Number: BC041629
• Gene Symbol: MAZ
• Gene ID (NCBI): 4150
• RRID: AB_2878805
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Oxid Med Cell Longev; TUG1/MAZ/FTH1 Axis Attenuates the Antiglioma Effect of Dihydroartemisinin by Inhibiting Ferroptosis; Authors - Hailong Gong
mouse	IF	Cell Rep; Multi-omics profiling visualizes dynamics of cardiac development and functions; Authors - Yayun Gu
human	WB	Genomics; PLOD1 promote proliferation and migration with glycolysis via the Wnt/β-catenin pathway in THCA; Authors - Wei Cong