• Featured Product
  • KD/KO Validated

MBD2 Polyclonal antibody

MBD2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 55200-1-AP

Synonyms

Demethylase, DMTase, MBD2, NY CO 41



Tested Applications

Positive WB detected inJurkat cells, human brain tissue, A431 cells, HEK-293 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

55200-1-AP targets MBD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name methyl-CpG binding domain protein 2
Calculated molecular weight 43 kDa
Observed molecular weight 43-45 kDa
GenBank accession numberNM_003927
Gene symbol MBD2
Gene ID (NCBI) 8932
RRIDAB_10852963
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.

Protocols

Product Specific Protocols
WB protocol for MBD2 antibody 55200-1-APDownload protocol
IHC protocol for MBD2 antibody 55200-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
  • KD Validated
mouseWB

Exp Cell Res

Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.

Authors - Ling Cheng
  • KO Validated
humanWB

Cell Rep

TRPS1 drives heterochromatic origin refiring and cancer genome evolution.

Authors - Jianguo Yang
humanWB

Genet Test Mol Biomarkers

The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.

Authors - Mei-Rong Liang
humanWB,IF

Gene

Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database

Authors - Jianjun Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Manfred (Verified Customer) (05-09-2022)

The antibody worked perfectly without any unspecific bands

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: PBMCs