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- KD/KO Validated
MBD2 Polyclonal antibody
MBD2 Polyclonal Antibody for IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
human and More (1)
Cat no : 55200-1-AP
Validation Data Gallery
|Positive WB detected in||Jurkat cells, A431 cells, HEK-293 cells, human brain tissue|
|Positive IHC detected in||human cervical cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
55200-1-AP targets MBD2 in WB, IHC,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||methyl-CpG binding domain protein 2|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||43-45 kDa|
|GenBank accession number||NM_003927|
|Gene ID (NCBI)||8932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.
|Product Specific Protocols|
|WB protocol for MBD2 antibody 55200-1-AP||Download protocol|
|IHC protocol for MBD2 antibody 55200-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
Exp Cell Res
Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.
TRPS1 drives heterochromatic origin refiring and cancer genome evolution.
Genet Test Mol Biomarkers
The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Manfred (Verified Customer) (05-09-2022)
The antibody worked perfectly without any unspecific bands