Validation Data Gallery
|Positive WB detected in
|Jurkat cells, human brain tissue, A431 cells, HEK-293 cells
|Positive IHC detected in
|human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:500-1:1000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
55200-1-AP targets MBD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Rabbit / IgG
|methyl-CpG binding domain protein 2
|Calculated molecular weight
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.
Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.
Exp Cell Res
Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.
TRPS1 drives heterochromatic origin refiring and cancer genome evolution.
Genet Test Mol Biomarkers
The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.
Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Manfred (Verified Customer) (05-09-2022)
The antibody worked perfectly without any unspecific bands