MBD2 Polyclonal antibody

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  WB analysis of Jurkat using 55200-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 55200-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 55200-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 55200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 55200-1-AP (MBD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 55200-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 55200-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55200-1-AP (MBD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

MBD2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55200-1-AP

Publications(7)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Demethylase, DMTase, MBD2, NY CO 41

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Tested Applications

• Positive WB detected in: Jurkat cells, human brain tissue, A431 cells, HEK-293 cells
• Positive IHC detected in: human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IF: See 1 publications below

Product Information

55200-1-AP targets MBD2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: methyl-CpG binding domain protein 2
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43-45 kDa
• GenBank Accession Number: NM_003927
• Gene Symbol: MBD2
• Gene ID (NCBI): 8932
• RRID: AB_10852963
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UBB5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD2, also named as DMTase, binds CpG islands in promoters where the DNA is methylated at position 5 of cytosine within CpG dinucleotides. MBD2 binds hemi-methylated DNA as well. It recruits histone deacetylases and DNA methyltransferases. MBD2 acts as transcriptional repressor and plays a role in gene silencing. (PMID:9774669) This antibody is specific to MBD2. It recognize isoform1 and isoform2 of MBD2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
mouse	WB	Exp Cell Res; Loss of MBD2 affects early T cell development by inhibiting the WNT signaling pathway.; Authors - Ling Cheng ; KO Validated
human	WB	Cell Rep; TRPS1 drives heterochromatic origin refiring and cancer genome evolution.; Authors - Jianguo Yang
human	WB	Genet Test Mol Biomarkers; The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.; Authors - Mei-Rong Liang
human	WB,IF	Gene; Exploring the importance of m5c in the diagnosis and subtype classification of COPD using the GEO database; Authors - Jianjun Wu
human	WB	Nucleic Acids Res; CARM1 hypermethylates the NuRD chromatin remodeling complex to promote cell cycle gene expression and breast cancer development; Authors - Xue Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Manfred (Verified Customer) (05-09-2022)

The antibody worked perfectly without any unspecific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: PBMCs