MBD4 Polyclonal antibody

• 

  WB analysis of human skin using 11270-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11270-1-AP (MBD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11270-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11270-1-AP (MBD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

MBD4 Polyclonal Antibody for WB, ELISA

Cat No. 11270-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:3.2.2.-, MED1, Methyl-CpG-binding domain protein 4, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4

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Tested Applications

• Positive WB detected in: human skin tissue, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

11270-1-AP targets MBD4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1822

  Product name: Recombinant human MBD4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 228-574 aa of BC011752

  Sequence: VRKPKGKVTILKGIPIKKTKKGCRKSCSGFVQSDSKRESVCNKADAESEPVAQKSQLDRTVCISDAGACGETLSVTSEENSLVKKKERSLSSGSNFCSEQKTSGIINKFCSAKDSEHNEKYEDTFLESEEIGTKVEVVERKEHLHTDILKRGSEMDNNCSPTRKDFTEDTIPRTQIERRKTSLYFSSKYNKEALSPPRRKAFKKWTPPRSPFNLVQETLFHDPWKLLIATIFLNRTSGKMAIPVLWKFLEKYPSAEVARTADWRDVSELLKPLGLYDLRAKTIVKFSDEYLTKQWKYPIELHGIGKYGNDSYRIFCVNEWKQVHPEDHKLNKYHDWLWENHEKLSLS

• Full Name: methyl-CpG binding domain protein 4
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC011752
• Gene Symbol: MBD4
• Gene ID (NCBI): 8930
• RRID: AB_2139871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95243
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD4, also named as MED1, is a 580 amino acid protein, which Interacts with MLH1. MBD4 as a mismatch-specific DNA N-glycosylase is involved in DNA repair and has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. MBD4 can also remove uracil or 5-fluorouracil in G:U mismatches. MBD4 exists some isoforms with MV 66 kDa and 30 kDa (The identification of a novel alternatively spliced form of the MBD4 DNA glycosylase).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.; Authors - Xiao-Hui Wang ; KD Validated
human	WB	Genet Test Mol Biomarkers; The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.; Authors - Mei-Rong Liang