Tested Applications
| Positive WB detected in | human plasma, rat plasma |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24207-1-AP targets MBL in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21508 Product name: Recombinant human MBL2 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 21-248 aa of BC069338 Sequence: ETVTCEDAQKTCPAVIACSSPGINGFPGKDGRDGTKGEKGEPGQGLRGLQGPPGKLGPPGNPGPSGSPGPKGQKGDPGKSPDGDSSLAASERKALQTEMARIKKWLTFSLGKQVGNKFFLTNGEIMTFEKVKALCVKFQASVATPRNAAENGAIQNLIKEEAFLGITDEKTEGQFVDLTGNRLTYTNWNEGEPNNAGSDEDCVLLLKNGQWNDVPCSTSHLAVCEFPI Predict reactive species |
| Full Name | mannose-binding lectin (protein C) 2, soluble (opsonic defect) |
| Calculated Molecular Weight | 248 aa, 26 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC069338 |
| Gene Symbol | MBL2 |
| Gene ID (NCBI) | 4153 |
| RRID | AB_2879458 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11226 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MBL antibody 24207-1-AP | Download protocol |
| WB protocol for MBL antibody 24207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MBL Polyclonal antibody (Cat# 24207-1-AP) has 7 citations across journals including Advanced Science, International Immunopharmacology, Scientific Reports, Oncology Reports, Cancer Control, Biochemical and Biophysical Research Communications, and medRxiv. Research fields span immune thrombocytopenia and exosome biology, neuronal injury and hypomyelination, CRP-mediated inflammation, breast cancer progression, hepatocellular carcinoma, unfolded protein response in silkworms, and muscular dystrophy biomarker identification. Applications include IF, WB, and IHC in human and rat samples.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Exosome-Mediated Lectin Pathway and Resistin-MIF-AA Metabolism Axis Drive Immune Dysfunction in Immune Thrombocytopenia | ||
Int Immunopharmacol Combined VEGF and bFGF loaded nanofiber membrane protects against neuronal injury and hypomyelination in a rat model of chronic cerebral hypoperfusion | ||
Sci Rep C-reactive protein (CRP) recognizes uric acid crystals and recruits proteases C1 and MASP1. | ||
Oncol Rep HIF‑1α and MBP1 are associated with the progression of breast cancer cells by repressing β‑catenin transcription. | ||
Cancer Control MiR-942-3p Promotes the Proliferation and Invasion of Hepatocellular Carcinoma Cells by Targeting MBL2.
| ||
Biochem Biophys Res Commun Characterization of a silkworm UFM1 homolog in regulating Bombyx mori unfolded protein response and nucleopolyhedrovirus replication |
