|Positive WB detected in||HeLa cells, A375 cells, mouse lung tissue, HepG2 cells|
|Positive IP detected in||HeLa cells, HepG2 cells|
|Positive IHC detected in||human ovary tumor tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Positive FC detected in||HUVEC cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:200-1:600|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17564-1-AP targets CD146/MCAM in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CD146/MCAM fusion protein Ag11762|
|Full Name||melanoma cell adhesion molecule|
|Calculated molecular weight||646 aa, 72 kDa|
|Observed molecular weight||113-120 kDa|
|GenBank accession number||BC056418|
|Gene ID (NCBI)||4162|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).
|Product Specific Protocols|
|WB protocol for CD146/MCAM antibody 17564-1-AP||Download protocol|
|IHC protocol for CD146/MCAM antibody 17564-1-AP||Download protocol|
|IF protocol for CD146/MCAM antibody 17564-1-AP||Download protocol|
|IP protocol for CD146/MCAM antibody 17564-1-AP||Download protocol|
|FC protocol for CD146/MCAM antibody 17564-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (09-28-2020)
This antibody recognises a band around the expected molecular weight, however this band did not respond to deglycosylation treatment. This strongly suggests the recognised band is another protein other than Mcam, because Mcam is glycosylated, and looking at the other Mcam antibody from the same brand (66153-1-g), it is clear that the band is responsive to PNGaseF treatment.