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  • KD/KO Validated

CD146/MCAM Polyclonal antibody

CD146/MCAM Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17564-1-AP

Synonyms

CD146, MCAM, MUC18



Tested Applications

Positive WB detected inHeLa cells, mouse lung tissue, A375 cells, HepG2 cells
Positive IP detected inHeLa cells, HepG2 cells
Positive IHC detected inhuman ovary tumor tissue, human brain tissue, human heart tissue, human kidney tissue, human lung tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, HeLa cells
Positive FC detected inHUVEC cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB
Immunohistochemistry (IHC)IHC : 1:200-1:600
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17564-1-AP targets CD146/MCAM in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD146/MCAM fusion protein Ag11762
Full Name melanoma cell adhesion molecule
Calculated molecular weight 646 aa, 72 kDa
Observed molecular weight 113-120 kDa
GenBank accession numberBC056418
Gene symbol MCAM
Gene ID (NCBI) 4162
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CD146, also known as melanoma cell adhesion molecule (MCAM) or MUC18, originally identified as a biomarker of melanoma progression, is a transmembrane glycoprotein of 113-130 kDa, belonging to the immunoglobulin (Ig) superfamily (PMID: 8378324; 25993332). Structurally, it consists of five Ig domains, a transmembrane domain, and a cytoplasmic region. In normal adult tissue, CD146 is primarily expressed by vascular endothelium and smooth muscle. CD146 is a key cell adhesion protein in vascular endothelial cell activity and angiogenesis, and has been used as marker of circulating endothelium cells (CECs) (PMID: 19356677). In addition to the membrane-anchored form of CD146, a soluble form of CD146 (sCD146, 105 kDa) has also been found in human plasma and in the supernatant of cultured human endothelial cells (PMID: 9462829; 19229070; 16374253; 14597988).

Protocols

Product Specific Protocols
WB protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IHC protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IF protocol for CD146/MCAM antibody 17564-1-APDownload protocol
IP protocol for CD146/MCAM antibody 17564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Biomaterials

Macropore design of tissue engineering scaffolds regulates mesenchymal stem cell differentiation fate.

Authors - W Benton Swanson
humanWB

Oncogene

The histone demethylase KDM3A, and its downstream target MCAM, promote Ewing Sarcoma cell migration and metastasis.

Authors - M Sechler
  • KD Validated
humanIF

Cell Prolif

Dental pulp stem cells-based therapy for the oviduct injury via immunomodulation and angiogenesis in vivo.

Authors - Lihua Luo
humanFC

Stem Cell Res Ther

Connective tissue growth factor promotes cementogenesis and cementum repair via Cx43/β-catenin axis

Authors - Zuping Wu
ratWB

J Proteome Res

Comparative Proteomic Analysis of Primary Schwann Cells and a Spontaneously Immortalized Schwann Cell Line RSC 96: A Comprehensive Overview with a Focus on Cell Adhesion and Migration Related Proteins.

Authors - Ji Yuhua Y
  • KD Validated
IF

Biochim Biophys Acta Mol Cell Biol Lipids

Salvianolic acid B ameliorates atherosclerosis via inhibiting YAP/TAZ/JNK signaling pathway in endothelial cells and pericytes.

Authors - Ying Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Boyan (Verified Customer) (09-28-2020)

This antibody recognises a band around the expected molecular weight, however this band did not respond to deglycosylation treatment. This strongly suggests the recognised band is another protein other than Mcam, because Mcam is glycosylated, and looking at the other Mcam antibody from the same brand (66153-1-g), it is clear that the band is responsive to PNGaseF treatment.

  • Applications: Western Blot