MCCC1 Polyclonal antibody

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• MCCC1 Polyclonal antibody (14861-1-AP)

MCCC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 14861-1-AP Copied!

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

MCC B, MCCA, MCCase subunit alpha, MCCC1

• 

  WB analysis of mouse liver using 14861-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse heart using 14861-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 14861-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14861-1-AP (MCCC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse liver using 14861-1-AP

  IP result of anti-MCCC1 (IP:14861-1-AP, 4ug; Detection:14861-1-AP 1:1000) with mouse liver tissue lysate 6000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 14861-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14861-1-AP (MCCC1 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 14861-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14861-1-AP (MCCC1 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse heart tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14861-1-AP targets MCCC1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6663

  Product name: Recombinant human MCCC1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-365 aa of BC004187

  Sequence: MAAASAVSVLLVAAERNRWHRLPSLLLPPRTWVWRQRTMKYTTATGRNITKVLIANRGEIACRVMRTAKKLGVQTVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLSMEKIIQVAKTSAAQAIHPGCGFLSENMEFAELCKQEGIIFIGPPPSAIRDMGIKSTSKSIMAAAGVPVVEGYHGEDQSDQCLKEHARRIGYPVMIKAVRGGGGKGMRIVRSEQEFQEQLESARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAPAPGIKSEVRKKLGEAAVRAAKAVNYVGAGTVEFIMDSKHNFCFMEMNTRLQVEHPVTEMITGTDLVEWQLRIAAG

• Full Name: methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 70-76 kDa
• GenBank Accession Number: BC004187
• Gene Symbol: MCCC1
• Gene ID (NCBI): 56922
• RRID: AB_2143798
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96RQ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.

Publications

What published studies show

The MCCC1 Polyclonal antibody (Cat# 14861-1-AP) has 4 citations published in Nature Medicine, Advanced Science, Life Sciences, and Biology (Basel). Research fields span cancer cell line metabolism, cisplatin-induced nephrotoxicity and antitumor efficacy, multidrug resistance in multiple myeloma, and TIA1 interactome mapping via mass spectrometry. Applications cited include WB and IHC in human and mouse samples.

Automatically generated from the 4 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Mouse	IHC	Nat Med; The landscape of cancer cell line metabolism.; Authors - Haoxin Li
mouse, human	WB	Adv Sci (Weinh); Icaritin Ameliorates Cisplatin-Induced Mitochondrial Metabolic Dysfunction-Associated Nephrotoxicity and Synergistically Potentiates Its Antitumor Efficacy.; Authors - Piao Luo
human	WB,IHC	Life Sci; Methylcrotonyl-CoA carboxylase subunit 1 (MCCA) regulates multidrug resistance in multiple myeloma; Authors - Yu Feng
human	WB	Biology (Basel); In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.; Authors - Olga Gourdomichali