MCCC1 Polyclonal antibody

MCCC1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30081-1-AP

Synonyms

DKFZp686B20267, FLJ25545, MCC B, MCCA, MCCase subunit alpha, MCCC1

---

Validation Data Gallery

• 

  WB analysis using 30081-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30081-1-AP (MCCC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HepG2 cells, mouse brain tissue, mouse heart tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30081-1-AP targets MCCC1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCCC1 fusion protein Ag32459
• Full Name: methylcrotonoyl-Coenzyme A carboxylase 1 (alpha)
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC004187
• Gene Symbol: MCCC1
• Gene ID (NCBI): 56922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCCC1 is the large subunit of 3-methylcrotonyl-CoA carboxylase. This enzyme functions as a heterodimer and catalyzes the carboxylation of 3-methylcrotonyl-CoA to form 3-methylglutaconyl-CoA. Mutations in this gene are associated with 3-Methylcrotonylglycinuria, an autosomal recessive disorder of leucine catabolism.