MCCC2 Polyclonal antibody

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• MCCC2 Polyclonal antibody (12117-1-AP)

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• KD/KO Validated

MCCC2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12117-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

3-methylcrotonyl-CoA carboxylase 2, 3-methylcrotonyl-CoA carboxylase non-biotin-containing subunit, 3-methylcrotonyl-CoA:carbon dioxide ligase subunit beta, EC:6.4.1.4, MCCase subunit beta

• 

  WB analysis of human liver using 12117-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12117-1-AP (MCCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse kidney using 12117-1-AP

  IP result of anti-MCCC2 (IP:12117-1-AP, 4ug; Detection:12117-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 12117-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12117-1-AP (MCCC2 antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12117-1-AP targets MCCC2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2767

  Product name: Recombinant human MCCC2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-329 aa of BC014897

  Sequence: MWAVLRLALRPCARASPAGPRAYHGDSVASLGTQPDLGSALYQENYKQMKALVNQLHERVEHIKLGGGEKARALHISRGKLLPRERIDNLIDPGSPFLELSQFAGYQLYDNEEVPGGGIITGIGRVSGVECMIIANDATVKGGAYYPVTVKKQLRAQEIAMQNRLPCIYLVDSGGAYLPRQADVFPDRDHFGRTFYNQAIMSSKNIAQVKAATGEEVSAEDLGGADLHCRKSGVSDHWALDDHHALHLTRKVVRNLNYQKKLDVTIEPSEEPLFPADELYGIVGANLKRSFDVREVIARIVDGSRFTEFKAFYGDTLVTGIKPLEKHKT

• Full Name: methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
• Calculated Molecular Weight: 563 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014897
• Gene Symbol: MCCC2
• Gene ID (NCBI): 64087
• RRID: AB_2143810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HCC0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCCC2(Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial) is also named as MCCB and belongs to the AccD/PCCB family. The putative 563-amino acid polypeptide has a calculated molecular mass of 61 kD and contains an N-terminal mitochondrial targeting sequence(PMID:11181649). It catalyzes carboxylation of 3 methylcrotonyl COA to form 3 methylglutaconyl-COA and is probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits. Defects in MCCC2 are the cause of methylcrotonoyl-CoA carboxylase 2 deficiency (MCC2D).

Publications

What published studies show

The MCCC2 Polyclonal antibody (Cat# 12117-1-AP) has 7 citations published in Cell Metabolism, Molecular Medicine, Cancer Cell International, Journal of Proteome Research, Kidney Research and Clinical Practice, Andrology, and Endocrine Connections. Research fields span mitochondrial metabolism under hypoxia, gastric cancer, hepatocellular carcinoma, prostate cancer proteomics, cisplatin-induced kidney injury, reproductive biology, and branched-chain amino acid catabolism in obesity.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Cell Metab; Mitochondrial VHL rewires cell metabolism in hypoxia.; Authors - Guobang Li ; KD Validated
mouse,human	WB,IF,CoIP	Mol Med; ECHDC2 inhibits the proliferation of gastric cancer cells by binding with NEDD4 to degrade MCCC2 and reduce aerobic glycolysis; Authors - Jiancheng He
human	WB	Cancer Cell Int; MCCC2 promotes HCC development by supporting leucine oncogenic function.; Authors - Yu-Yan Chen ; KO Validated
human	WB,IHC	J Proteome Res; Profiling protein markers associated with lymph node metastasis in prostate cancer by DIGE-based proteomics analysis.; Authors - Pang Jun J
		Kidney Res Clin Pract; Methylcrotonyl-CoA carboxylase 2 supports leucine catabolism to promote mitochondrial biogenesis and alleviate cisplatin-induced acute kidney injury.; Authors - Hu Li
	WB	Andrology; Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.; Authors - J Dai