MCCC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

MCCC2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 12117-1-AP

Synonyms

MCCase subunit beta, MCCB, MCCC2

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Validation Data Gallery

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  WB analysis of human liver using 12117-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12117-1-AP (MCCC2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 12117-1-AP

  IP result of anti-MCCC2 (IP:12117-1-AP, 4ug; Detection:12117-1-AP 1:300) with mouse kidney tissue lysate 4000ug.

• 

  IHC staining of human pancreas cancer using 12117-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12117-1-AP (MCCC2 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human liver tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

12117-1-AP targets MCCC2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCCC2 fusion protein Ag2767
• Full Name: methylcrotonoyl-Coenzyme A carboxylase 2 (beta)
• Calculated Molecular Weight: 563 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC014897
• Gene Symbol: MCCC2
• Gene ID (NCBI): 64087
• RRID: AB_2143810
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCCC2(Methylcrotonoyl-CoA carboxylase beta chain, mitochondrial) is also named as MCCB and belongs to the AccD/PCCB family. The putative 563-amino acid polypeptide has a calculated molecular mass of 61 kD and contains an N-terminal mitochondrial targeting sequence(PMID:11181649). It catalyzes carboxylation of 3 methylcrotonyl COA to form 3 methylglutaconyl-COA and is probably a dodecamer composed of six biotin-containing alpha subunits and six beta subunits. Defects in MCCC2 are the cause of methylcrotonoyl-CoA carboxylase 2 deficiency (MCC2D).

Publications

Species	Application	Title
human	WB,IHC	J Proteome Res; Profiling protein markers associated with lymph node metastasis in prostate cancer by DIGE-based proteomics analysis.; Authors - Pang Jun J
human	WB	Cancer Cell Int; MCCC2 promotes HCC development by supporting leucine oncogenic function.; Authors - Yu-Yan Chen ; KO Validated
	WB	Andrology; Nicotine elevates sperm motility and induces Pfn1 promoter hypomethylation in mouse testis.; Authors - J Dai