MCM3 Polyclonal antibody

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• MCM3 Polyclonal antibody (15597-1-AP)

• KD/KO Validated

MCM3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 15597-1-AP Copied!

Publications (31)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, RIP, ELISA

Synonyms

DNA polymerase alpha holoenzyme-associated protein P1, EC:3.6.4.12, P1 MCM3, p102, P1-MCM3

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  WB analysis using 15597-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15597-1-AP (MCM3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HeLa using 15597-1-AP

  WB result of MCM3 antibody (15597-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM3 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 15597-1-AP

  IP result of anti-MCM3 (IP:15597-1-AP, 4ug; Detection:15597-1-AP 1:6000) with HeLa cells lysate 1280 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 15597-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15597-1-AP (MCM3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells, Raji cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human colon cancer tissue, mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15597-1-AP targets MCM3 in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7966

  Product name: Recombinant human MCM3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 201-503 aa of BC001626

  Sequence: IQEMPEKAPAGQLPRSVDVILDDDLVDKAKPGDRVQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDAQPSFSAEDIAKIKKFSKTRSKDIFDQLAKSLAPSIHGHDYVKKAILCLLLGGVERDLENGSHIRGDINILLIGDPSVAKSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRTAIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMDPEQDREISDHVLR

• Full Name: minichromosome maintenance complex component 3
• Calculated Molecular Weight: 91 kDa
• Observed Molecular Weight: 102-105 kDa
• GenBank Accession Number: BC001626
• Gene Symbol: MCM3
• Gene ID (NCBI): 4172
• RRID: AB_2141973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P25205
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCM3, also named as HCC5, P1-MCM3, p102, RLF subunit beta and RLFB, belongs to the MCM family. MCM3 is essential for the initiation of DNA replication and is involved in ensuring that DNA replication is initiated precisely once per cell cycle(PMID: 33093917). MCM3 has 2 isoforms with the molecular mass of 91 and 96 kDa. The overexpression of MCM3 is related to proliferation of tumor cells(PMID: 31186514).

Publications

What published studies show

The MCM3 Polyclonal antibody (Cat# 15597-1-AP) has 31 citations across journals including Nature Communications, Molecular Cell, Advanced Science, eLife, Oncogene, Biomaterials, and Aging Cell. Research fields span DNA replication, cell cycle regulation, and multiple cancers including glioblastoma, hepatocellular carcinoma, lung cancer, gastric cancer, and breast cancer. Additional studies cover chromatin organization, angiogenesis, aging-related osteoporosis, and epigenetic regulation.

Automatically generated from the 31 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Commun; ICAM1 initiates CTC cluster formation and trans-endothelial migration in lung metastasis of breast cancer.; Authors - Rokana Taftaf
human	WB,RIP	Mol Cell; Alu repeat-containing RNAs spatially organize actively transcribed genomic regions around nuclear speckles.; Authors - Siqi Liu
human	WB	Adv Sci (Weinh); Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage; Authors - Jiaxi Zhang
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	WB	Sci China Life Sci; SETD3-mediated histidine methylation of MCM7 regulates DNA replication by facilitating chromatin loading of MCM; Authors - Hongguo Duan
		Int J Biol Macromol; A cancer-specific pipeline uncovers MCM3 as a driver of glioblastoma progression via suppression of the Wnt pathway.; Authors - Fei Wang