MCM4 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• MCM4 Polyclonal antibody (13043-1-AP)

• KD/KO Validated

MCM4 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 13043-1-AP

Publications(27)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

CDC21, CDC54, EC:3.6.4.12, hCdc21, P1 CDC21

• 

  WB analysis of HeLa using 13043-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 13043-1-AP

  WB result of MCM4 antibody (13043-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MCM4 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 13043-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 13043-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HL-60 using 13043-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13043-1-AP (MCM4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 13043-1-AP

  IP result of anti-MCM4 (IP:13043-1-AP, 4ug; Detection:13043-1-AP 1:600) with HEK-293 cells lysate 1200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat colon using 13043-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 13043-1-AP (MCM4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse testis using 13043-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using MCM4 antibody (13043-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse testis using 13043-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using MCM4 antibody (13043-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

---

Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HL-60 cells, human liver tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human colon cancer tissue, mouse testis tissue, rat colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13043-1-AP targets MCM4 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3703

  Product name: Recombinant human MCM4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 515-859 aa of BC031061

  Sequence: SKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKT

• Full Name: minichromosome maintenance complex component 4
• Calculated Molecular Weight: 863 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC031061
• Gene Symbol: MCM4
• Gene ID (NCBI): 4173
• RRID: AB_2142293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P33991
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.This antibody is a rabbit polyclonal antibody raised against the C-terminal 350 aa sequence of MCM4 protein.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IF	Nat Commun; CRL4(DCAF12) regulation of MCMBP ensures optimal licensing of DNA replication.; Authors - Anoop Kumar Yadav
mouse	WB	Nat Commun; DNA replication fork speed acts as a pacer in cortical neurogenesis.; Authors - Jianhong Wang
human	WB,IHC	Adv Sci (Weinh); Topoisomerase I Inhibition in ETV4-overexpressed Non-Small Cell Lung Cancer Promotes Replication and Transcription Mediated R-Loop Accumulation and DNA Damage; Authors - Jiaxi Zhang
human	WB	Sci Adv; Small-molecule OPA1 inhibitors reverse mitochondrial adaptations to overcome therapy resistance in acute myeloid leukemia.; Authors - Sofia La Vecchia
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
mouse	WB	Nat Struct Mol Biol; A new vertebrate SUMO enzyme family reveals insights into SUMO-chain assembly.; Authors - Nathalie Eisenhardt