MCM4 Monoclonal antibody, PBS Only

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  WB analysis using 67103-1-Ig (same clone as 67103-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 67103-1-Ig (same clone as 67103-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 67103-1-Ig (same clone as 67103-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67103-1-Ig (MCM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 67103-1-Ig (same clone as 67103-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MCM4 antibody (67103-1-Ig, Clone: 2H2A1 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 67103-1-PBS in a different storage buffer formulation.

MCM4 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 67103-1-PBS

Clone No.2H2A1

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

P1 CDC21, hCdc21, EC:3.6.4.12, CDC54, CDC21

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Product Information

67103-1-PBS targets MCM4 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28642

  Product name: Recombinant human MCM4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 515-859 aa of BC031061

  Sequence: SKSQLLQYVYNLVPRGQYTSGKGSSAVGLTAYVMKDPETRQLVLQTGALVLSDNGICCIDEFDKMNESTRSVLHEVMEQQTLSIAKAGIICQLNARTSVLAAANPIESQWNPKKTTIENIQLPHTLLSRFDLIFLMLDPQDEAYDRRLAHHLVALYYQSEEQAEEELLDMAVLKDYIAYAHSTIMPRLSEEASQALIEAYVDMRKIGSSRGMVSAYPRQLESLIRLAEAHAKVRLSNKVEAIDVEEAKRLHREALKQSATDPRTGIVDISILTTGMSATSRKRKEELAEALKKLILSKGKTPALKYQQLFEDIRGQSDIAITKDMFEEALRALADDDFLTVTGKT

• Full Name: minichromosome maintenance complex component 4
• Calculated Molecular Weight: 863 aa, 97 kDa
• Observed Molecular Weight: 97 kDa
• GenBank Accession Number: BC031061
• Gene Symbol: MCM4
• Gene ID (NCBI): 4173
• RRID: AB_2882408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P33991
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DNA replication licensing factor MCM4 (MCM4), also named Cdc21, acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.