MCOLN1 Polyclonal antibody, PBS Only

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• MCOLN1 Polyclonal antibody, PBS Only (15291-1-PBS)

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  WB analysis using 15291-1-AP (same clone as 15291-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15291-1-AP (MCOLN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15291-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15291-1-AP (same clone as 15291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15291-1-AP (MCOLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15291-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 15291-1-AP (same clone as 15291-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15291-1-AP (MCOLN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15291-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 15291-1-AP (same clone as 15291-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using MCOLN1 antibody (15291-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15291-1-PBS in a different storage buffer formulation.

MCOLN1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 15291-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

MG 2, ML1, ML4, MLIV, MST080

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Product Information

15291-1-PBS targets MCOLN1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7493

  Product name: Recombinant human MCOLN1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 90-299 aa of BC005149

  Sequence: AVTFREENTIAFRHLFLLGYSDGADDTFAAYTREQLYQAIFHAVDQYLALPDVSLGRYAYVRGGGDPWTNGSGLALCQRYYHRGHVDPANDTFDIDPMVVTDCIQVDPPERPPPPPSDDLTLLESSSSYKNLTLKFHKLVNVTIHFRLKTINLQSLINNEIPDCYTFSVLITFDNKAHSGRIPISLETQAHIQECKHPSVFQHGDNSFRL

• Full Name: mucolipin 1
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC005149
• Gene Symbol: MCOLN1
• Gene ID (NCBI): 57192
• RRID: AB_2935438
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9GZU1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MCOLN1, also named MLIV, ML4, MG-2, and Mucolipidin, belongs to the transient receptor (TC 1.A.4) family, Polycystin subfamily, and MCOLN1 sub-subfamily. It is playing a role in the endocytic pathway and in the control of membrane trafficking of proteins and lipids. MCOLN1 may play a major role in Ca2+ transport regulating lysosomal exocytosis. It is an autosomal recessive lysosomal storage disorder characterized by severe psychomotor retardation and ophthalmologic abnormalities, including corneal opacity, retinal degeneration, and strabismus.