MCOLN3 Recombinant antibody

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  WB analysis using 86202-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86202-1-RR (MCOLN3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

MCOLN3 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 86202-1-RR

Clone No.250799F8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Mucolipin 3, Transient receptor potential channel mucolipin 3, TRP ML3, TRPML3

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Tested Applications

• Positive WB detected in: A375 cells, HeLa cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86202-1-RR targets MCOLN3 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MCOLN3 fusion protein Ag4962
• Full Name: mucolipin 3
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC060765
• Gene Symbol: MCOLN3
• Gene ID (NCBI): 55283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8TDD5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MCOLN3 (Mucolipin 3) is a member of the TRPML (Transient Receptor Potential Mucolipin) gene family and encodes a non-selective cation channel protein that is primarily localized on the membranes of endosomes and lysosomes. MCOLN3 promotes endosome acidification, regulates membrane transport and fusion, and is involved in the autophagy process. Additionally, MCOLN3 is involved in the regulation of intracellular calcium (Ca²⁺) and iron (Fe²⁺) ion homeostasis, maintains lysosomal membrane potential, promotes endosome-lysosome fusion and material transport (such as autophagy), and may also be involved in neuronal signaling and immune regulation.