MCP-1/CCL2 Recombinant monoclonal antibody, PBS Only (Detector)

• 

  Sandwich ELISA sample test of MP00938-1

  Urine of eight individual healthy human donors was measured. The human MCP-1 concentration of detected samples was determined to be 385.1 pg/mL with a range of 106.1 - 999.8 pg/mL.

• 

  Sandwich ELISA sample test of MP00938-1

  Human peripheral blood mononuclear cells (1 x 10^6 cells/mL) were cultured in RPMI-1640 supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were unstimulated or stimulated with 10 μg/mL PHA. Aliquots of the cell culture supernates were removed on days 3 and 5 and assayed for levels of human MCP-1.

• 

  Sandwich ELISA sample test of MP00938-1

  Serum of sixteen individual healthy human donors was measured. The human MCP-1 concentration of detected samples was determined to be 595.9 pg/mL with a range of 193.4 - 1,105.3 pg/mL.

• 

  Sandwich ELISA standard curve of MP00938-1

  Sandwich ELISA standard curve of MP00938-1, Human MCP-1 Recombinant Matched Antibody Pair - PBS only. 80847-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg31375. 80847-7-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL

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  Cytometric bead array standard curve of MP00938-1

  Cytometric bead array standard curve of MP00938-1, MCP-1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80847-4-PBS. Detection antibody: 80847-7-PBS. Standard: Eg31375. Range: 0.313-20 ng/mL.

MCP-1/CCL2 Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 80847-7-PBS

Clone No.241165G2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

CCL2, MCP1, MCP-1, C C motif chemokine 2, C-C motif chemokine 2

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Product Information

80847-7-PBS targets MCP-1/CCL2 as part of a matched antibody pair:

MP00938-1: 80847-4-PBS capture and 80847-7-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg31375

  Product name: Recombinant Human MCP-1/CCL2 protein (Myc Tag, His Tag)

  Source: mammalian cells-derived, pHZ-KIsec

  Tag: Myc & 6*His

  Domain: 24-99 aa of BC009716

  Sequence: QPDAINAPVTCCYNFTNRKISVQRLASYRRITSSKCPKEAVIFKTIVAKEICADPKQKWVQDSMDHLDKQTQTPKT

• Full Name: chemokine (C-C motif) ligand 2
• Calculated Molecular Weight: 99 aa, 11 kDa
• GenBank Accession Number: BC009716
• Gene Symbol: MCP-1/CCL2
• Gene ID (NCBI): 6347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13500
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.