M-CSF Polyclonal antibody

M-CSF Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, Cell treatment, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 14779-1-AP

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF

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Validation Data Gallery

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Tested Applications

Recommended dilution

Application	Dilution
Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 8 publications below
• IF: See 1 publications below

Product Information

14779-1-AP targets M-CSF in WB, IF, Cell treatment, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: M-CSF fusion protein Ag6294
• Full Name: colony stimulating factor 1 (macrophage)
• Calculated molecular weight: 60 kDa
• Observed molecular weight: 70 kDa
• GenBank accession number: BC021117
• Gene symbol: CSF1
• Gene ID (NCBI): 1435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Colony stimulating factor-1 (CSF-1) is a homodimeric glycoprotein that humorally regulates the proliferation and differentiation of mononuclear phagocytic cells and locally regulates cells of the female reproductive tract. Alternative splicing of the human CSF-1 mRNA leads to alternative expression of the CSF-1 homodimer as a secreted glycoprotein or as a membrane-spanning molecule with cell surface biological activity. CSF-1 is predominantly secreted as highly sulfated species of 375- and 250-kDa with a smaller amount of a 100-kDa species. The three predominant CSF-1 species were shown to be an 80-kDa homodimer, an 80-kDa/50-kDa heterodimer, and a 50-kDa homodimer. (PMID: 1733926) This antibody could recognieze 60 kDa, 48 kDa, 29 kDa isoforms.

Publications

Species	Application	Title
mouse	WB	J Control Release; Chemoattractants driven and microglia based biomimetic nanoparticle treating TMZ-resistant glioblastoma multiforme.; Authors - Sai Qiao
mouse	WB,Cell treatment	J Nanobiotechnology; Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM.; Authors - Bao Liu
	WB	Biomed Pharmacother; Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.; Authors - Xiaokang Jia
human	WB	Int J Biol Sci; Macrophage Colony-stimulating Factor Mediates the Recruitment of Macrophages in Triple negative Breast Cancer.; Authors - Xiaoqing Lu
human,mouse	WB	Invest Ophthalmol Vis Sci; CSF1/CSF1R-mediated Crosstalk Between Choroidal Vascular Endothelial Cells and Macrophages Promotes Choroidal Neovascularization.; Authors - Yamei Zhou
mouse	WB	Front Physiol; Heat Stress Modulates a Placental Immune Response Associated With Alterations in the Development of the Fetal Intestine and Its Innate Immune System in Late Pregnant Mouse.; Authors - Huiduo Guo