M-CSF Polyclonal antibody

M-CSF Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 14779-1-AP

Synonyms

CSF 1, CSF1, Lanimostim, M CSF, MCSF, M-CSF



Tested Applications

Recommended dilution

ApplicationDilution
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 8 publications below

Product Information

14779-1-AP targets M-CSF in WB, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen M-CSF fusion protein Ag6294
Full Name colony stimulating factor 1 (macrophage)
Calculated molecular weight 60 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC021117
Gene symbol CSF1
Gene ID (NCBI) 1435
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Colony stimulating factor-1 (CSF-1) is a homodimeric glycoprotein that humorally regulates the proliferation and differentiation of mononuclear phagocytic cells and locally regulates cells of the female reproductive tract. Alternative splicing of the human CSF-1 mRNA leads to alternative expression of the CSF-1 homodimer as a secreted glycoprotein or as a membrane-spanning molecule with cell surface biological activity. CSF-1 is predominantly secreted as highly sulfated species of 375- and 250-kDa with a smaller amount of a 100-kDa species. The three predominant CSF-1 species were shown to be an 80-kDa homodimer, an 80-kDa/50-kDa heterodimer, and a 50-kDa homodimer. (PMID: 1733926) This antibody could recognieze 60 kDa, 48 kDa, 29 kDa isoforms.

Publications

SpeciesApplicationTitle
mouseWB

Neuro Oncol

Colony stimulating factor 1 receptor inhibition delays recurrence of glioblastoma after radiation by altering myeloid cell recruitment and polarization.

Authors - Jason H Stafford
humanWB

J Exp Clin Cancer Res

Tumor cell-derived SPON2 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by activating PYK2 in CRC.

Authors - Chengmei Huang
mouseWB

Front Physiol

Heat Stress Modulates a Placental Immune Response Associated With Alterations in the Development of the Fetal Intestine and Its Innate Immune System in Late Pregnant Mouse.

Authors - Huiduo Guo
human,mouseWB

Invest Ophthalmol Vis Sci

CSF1/CSF1R-mediated Crosstalk Between Choroidal Vascular Endothelial Cells and Macrophages Promotes Choroidal Neovascularization.

Authors - Yamei Zhou
humanWB

Int J Biol Sci

Macrophage Colony-stimulating Factor Mediates the Recruitment of Macrophages in Triple negative Breast Cancer.

Authors - Xiaoqing Lu
WB

Biomed Pharmacother

Anti-osteoporotic effects of alisol C 23-acetate via osteoclastogenesis inhibition.

Authors - Xiaokang Jia