M-CSF Recombinant monoclonal antibody

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• M-CSF Recombinant monoclonal antibody (84481-4-RR)

M-CSF Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84481-4-RR Copied!

Clone No. 241743C5

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Synonyms

M CSF, Lanimostim, CSF-1, CSF1, CSF 1

• 

  WB analysis using 84481-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84481-4-RR (MCSF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity and Kinetic Characterization of 84481-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84481-4-RR against Human M-CSF were performed. The affinity constant is 11.4 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, THP-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84481-4-RR targets M-CSF in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: colony stimulating factor 1 (macrophage)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC021117
• Gene Symbol: M-CSF
• Gene ID (NCBI): 1435
• RRID: AB_3743565
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09603
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

M-CSF plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes. M-CSF promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. It also plays an important role in the regulation of osteoclast proliferation and differentiation, the regulation of bone resorption, and is required for normal bone development. On the cellular level, M-CSF promotes reorganization of the actin cytoskeleton, regulates formation of membrane ruffles, cell adhesion and cell migration and plays a role in lipoprotein clearance (PMID: 29734839; 29679908; 28202039)

Publications

What published studies show

The M-CSF Recombinant monoclonal antibody (241743C5), catalog number 84481-4-RR, has 2 total citations. One appears in Scientific Reports, studying CSF1's role in inflammation and apoptosis during intervertebral disc degeneration (WB, rat). The other is in bioRxiv, examining cross-subgenus hybridization between Leishmania and Sauroleishmania in the context of parasite genomic compatibility and transcriptomic adaptation. The associated research fields span musculoskeletal biology and parasitology.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Sci Rep; CSF1 regulates inflammation and apoptosis in intervertebral disc degeneration.; Authors - Fengyuan Zhang ; KD Validated
		bioRxiv; Cross-subgenus hybridization between Leishmania and Sauroleishmania informs on parasite genomic compatibility and transcriptomic adaptation; Authors - Viviane Noll Louzada-Flores