MD-1 Polyclonal antibody

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• MD-1 Polyclonal antibody (34213-1-AP)

• 

  WB analysis using 34213-1-AP

  Rat spleen tissue were subjected to SDS PAGE followed by western blot with 34213-1-AP (MD-1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of rat spleen using 34213-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 34213-1-AP (MD-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat spleen using 34213-1-AP

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 34213-1-AP (MD-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MD-1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 34213-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Ly86, Ly-86, Lymphocyte antigen 86, Md1, Protein MD-1

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Tested Applications

• Positive WB detected in: rat spleen tissue
• Positive IHC detected in: rat spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

34213-1-AP targets MD-1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg6369

  Product name: Recombinant mouse MD-1 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 20-162 aa of NM_010745.2

  Sequence: DHGSENGWPKHTACNSGGLEVVYQSCDPLQDFGLSIDQCSKQIQSNLNIRFGIILRQDIRKLFLDITLMAKGSSILNYSYPLCEEDQPKFSFCGRRKGEQIYYAGPVNNPGLDVPQGEYQLLLELYNENRATVACANATVTSS

• Full Name: lymphocyte antigen 86
• Calculated Molecular Weight: 18 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_010745.2
• Gene Symbol: Ly86
• Gene ID (NCBI): 17084
• RRID: AB_3743173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O88188
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphocyte antigen 86 (LY86), also known as myeloid differentiation protein 1 (MD1), encodes a secreted glycoprotein that participates in inflammation, humoral immune response, apoptosis, and signal transduction. LY86 is widely expressed in cardiac tissues and predominantly by B cells and macrophages. (PMID: 38822281)