MDM2 Monoclonal antibody

MDM2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

2A6C9

Cat no : 66511-1-Ig

Synonyms

Double minute 2 protein, hdm2, HDMX, MDM2, Oncoprotein Mdm2, p53 binding protein Mdm2



Tested Applications

Positive WB detected inMCF-7 cells, A549 cells, rat brain tissue, mouse brain tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Sample-dependent, check data in validation data gallery

Published Applications

CoIPSee 1 publications below

Product Information

66511-1-Ig targets MDM2 in WB, IHC, CoIP, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name Mdm2 p53 binding protein homolog (mouse)
Calculated molecular weight 55 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberNM_002392
Gene symbol MDM2
Gene ID (NCBI) 4193
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the cellular localization of MDM2?

MDM2 is predominantly localized in the nucleoplasm.

What is the tissue specificity of MDM2?

MDM2 is non-specific, expressing ubiquitously in the brain, heart, kidney, skin, and other tissues. Certain 

isoforms only express in cancerous cells and not normal tissue.

With what other molecules does MDM2 interact?

Most notably, the oncogene protein MDM2 regulates transcription factor p53 by binding its transcriptional 

activation domain and by E3-ubiquitin ligase activity. Ribosome proteins rpL5, rpL11, and rpL23 also bind to 

MDM2, inhibiting E3 ubiquitin ligase activity and promoting the p53 pathway.

What competes with MDM2 activity?

Nutilin-3 was designed to compete with MDM2 in binding with p53, inducing activation of the p53 pathway.

What is the role of ubiquitination in MDM2 activity?

While p53 is also affected by other post-translational modifications, its stability is determined by ubiquitination 

mediation of MDM2, suppressing p53 transcriptional regulation and leading to the proteosomal degradation of 

p53.

How is MDM2 associated with cancer?

MDM2 inhibits the activity of the tumor-suppressive protein p53, which serves as an important factor in 

promoting DNA repair or activating apoptotic pathways. Loss of p53 is associated with tumor development 

and growth.

What is the human ortholog of MDM2?

Human double-minute type 2 (HDM2)

In what cellular circumstances does the p53-MDM2 complex dissociate?

Cellular stress or DNA damage result in the phosphorylation and activation of p53. Phosphorylation also 

prevents p53-MDM2 interaction.

Where is the gene located?

Hdm2 is located on human 12q15.

Mdm2 is located on murine 10 D2.

PMIDs: 24309898, 29584707, 9840926, 28714398


Protocols

Product Specific Protocols
WB protocol for MDM2 antibody 66511-1-IgDownload protocol
IHC protocol for MDM2 antibody 66511-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Oncogenesis

MeCP2 facilitates breast cancer growth via promoting ubiquitination-mediated P53 degradation by inhibiting RPL5/RPL11 transcription.

Authors - DongDong Tong