MDMX/MDM4 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 84534-5-RR (same clone as 84534-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary cancer using 84534-5-RR (same clone as 84534-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84534-5-RR (MDMX/MDM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84534-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84534-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84534-5-RR against Human MDMX/MDM4 were performed. The affinity constant is 0.247 nM.

MDMX/MDM4 Uni-rAb^® Recombinant Antibody for WB, IHC, Indirect ELISA

Cat No. 84534-5-PBS

Clone No.241936D11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

MDMX, MDM4, p53-binding protein Mdm4, Mdm2-like p53-binding protein, HDMX

---

Product Information

84534-5-PBS targets MDMX/MDM4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag26678

  Product name: Recombinant human MDM4 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-332 aa of BC067299

  Sequence: MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYS

• Full Name: Mdm4 p53 binding protein homolog (mouse)
• Calculated Molecular Weight: 490 aa, 55 kDa
• Observed Molecular Weight: 70-76 kDa
• GenBank Accession Number: BC067299
• Gene Symbol: MDMX/MDM4
• Gene ID (NCBI): 4194
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O15151
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing.