Tested Applications
| Positive WB detected in | COLO 320 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15196-1-AP targets MED10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7336 Product name: Recombinant human MED10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-135 aa of BC003353 Sequence: MAEKFDHLEEHLEKFVENIRQLGIIVSDFQPSSQAGLNQKLNFIVTGLQDIDKCRQQLHDITVPLEVFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIRGEDHPPS Predict reactive species |
| Full Name | mediator complex subunit 10 |
| Calculated Molecular Weight | 16 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC003353 |
| Gene Symbol | MED10 |
| Gene ID (NCBI) | 84246 |
| RRID | AB_2878117 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BTT4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED10, also named as L6, TRG17, or TRG20, is a 135 amino acid protein, which belongs to the Mediator complex subunit 10 family. MED10 localizes in the nucleus and as a component of the Mediator complex, a coactivator is involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Multiprotein mediator complex acting as a coactivator is required for activation of RNA polymerase II transcription by DNA bound transcription factors.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED10 antibody 15196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MED10 Polyclonal antibody (Cat# 15196-1-AP) has 1 total citation. It appears in the Journal of Virology (J Virol), in a 2013 study examining how Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. The cited application is Western Blot in human samples, placing the research field in virology and transcriptional regulation.
| Species | Application | Title |
|---|---|---|
J Virol Adenovirus L-E1A activates transcription through mediator complex-dependent recruitment of the super elongation complex. |
Reviews
What customers say
The single available review rates this antibody 3 out of 5 stars. The reviewer used it for Western Blot at a 1:500 dilution on embryo heart tissue and reported observing a band around 15 kDa. Overall, the feedback suggests the antibody produced a detectable signal at the expected molecular weight, though the moderate rating indicates the result may not have been fully satisfactory.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
SITING (Verified Customer) (04-02-2021) | can see band aroun 15kd
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