|Positive WB detected in||mouse liver tissue|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
20028-1-AP targets MED12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||mediator complex subunit 12|
|Calculated molecular weight||243 kDa|
|Observed molecular weight||243 kDa|
|GenBank accession number||NM_005120|
|Gene ID (NCBI)||9968|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.
Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma.
MED12 Regulates Smooth Muscle Cell Functions and Participates in the Development of Aortic Dissection.
Pathol Res Pract
Cyclin-dependent kinase 8 is an independent prognosticator in uterine leiomyosarcoma.