MED12 Polyclonal antibody

MED12 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 20028-1-AP

Synonyms

ARC240, CAG repeat protein 45, CAGH45, FGS1, HOPA, KIAA0192, MED12, mediator complex subunit 12, OKS, OPA containing protein, OPA1, TNRC11, TRAP230



Tested Applications

Positive WB detected inmouse liver tissue
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20028-1-AP targets MED12 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name mediator complex subunit 12
Calculated molecular weight 243 kDa
Observed molecular weight 243 kDa
GenBank accession numberNM_005120
Gene symbol MED12
Gene ID (NCBI) 9968
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mediator of RNA polymerase II transcription subunit 12 (MED12), also named Activator-recruited cofactor 240 kDa component, Thyroid hormone receptor-associated protein complex 230 kDa component. MED12 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. This subunit may specifically regulate transcription of targets of the Wnt signaling pathway and SHH signaling pathway. Molecular weight of MED12 is about 240kd.This antibody is specific to MED12.

Protocols

Product Specific Protocols
WB protocol for MED12 antibody 20028-1-APDownload protocol
IF protocol for MED12 antibody 20028-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Mod Pathol

MED12 and HMGA2 mutations: two independent genetic events in uterine leiomyoma and leiomyosarcoma.

Authors - Elizabeth Bertsch
mouseWB

EMBO J

Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.

Authors - Lihua Wang