MED12L Polyclonal antibody

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  WB analysis of mouse liver using 20047-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20047-1-AP (MED12L antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 20047-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MED12L antibody (20047-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

MED12L Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 20047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IF/ICC, ELISA

Synonyms

KIAA1635, Mediator complex subunit 12-like protein, Mediator of RNA polymerase II transcription subunit 12-like protein, PRO0314, Thyroid hormone receptor-associated-like protein

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20047-1-AP targets MED12L in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mediator complex subunit 12-like
• Calculated Molecular Weight: 240 kDa
• Observed Molecular Weight: 260-280 kDa
• GenBank Accession Number: NM_053002
• Gene Symbol: MED12L
• Gene ID (NCBI): 116931
• RRID: AB_10734594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q86YW9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED12L, also named as KIAA1635, TNRC11L, TRALP and TRALPUSH, belongs to the Mediator complex subunit 12 family. MED12L may be a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. MED12L is a mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. MED12L is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. The antibody can recognize all the isoform of MED12L. The antibody has no cross reaction to MED12.