MED17 Polyclonal antibody

• 

  WB analysis of human liver using 11505-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11505-1-AP (MED17 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

MED17 Polyclonal Antibody for WB, ELISA

Cat No. 11505-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

ARC77, CRSP complex subunit 6, CRSP6, CRSP77, DRIP77, DRIP80, MED17, mediator complex subunit 17, TRAP80

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Tested Applications

• Positive WB detected in: human liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11505-1-AP targets MED17 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag2057

  Product name: Recombinant human MED17 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 296-651 aa of BC021101

  Sequence: EAAQNVLLCKEIFAQLSREAVQIKSQVPHIVVKNQIISQPFPSLQLSISLCHSSNDKKSQKFATEKQCPEDHLYVLEHNLHLLIREFHKQTLSSIMMPHPASAPFGHKRMRLSGPQAFDKNEINSLQSSEGLLEKIIKQAKHIFLRSRAAATIDSLASRIEDPQIQAHWSNINDVYESSVKVLITSQGYEQICKSIQLQLNIGVEQIRVVHRDGRVITLSYQEQELQDFLLSQMSQHQVHAVQQLAKVMGWQVLSFSNHVGLGPIESIGNASAITVASPSGDYAISVRNGPESGSKIMVQFPRNQCKDLPKSDVLQDNKWSHLRGPFKEVQWNKMEGRNFVYKMELLMSALSPCLL

• Full Name: mediator complex subunit 17
• Calculated Molecular Weight: 651 aa, 73 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC021101
• Gene Symbol: MED17
• Gene ID (NCBI): 9440
• RRID: AB_2141998
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NVC6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Nat Commun; Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.; Authors - Haining Zhou
human	WB	Nat Commun; Multiple structures of RNA polymerase II isolated from human nuclei by ChIP-CryoEM analysis; Authors - Tomoya Kujirai