Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13418-1-AP targets MED19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4165 Product name: Recombinant human MED19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-194 aa of BC037223 Sequence: MENFTALFGAQADPPPPPTALGFGPGKPPPPPPPPAGGGPGTAPPPTAATAPPGADKSGAGCGPFYLMRELPGSTELTGSTNLITHYNLEQAYNKFCGKKVKEKLSNFLPDLPGMIDLPGSHDNSSLRSLIEKPPILSSSFNPITGTMLAGFRLHTGPLPEQCRLMHIQPPKKKNKHKHKQSRTQDPVPPGKPS Predict reactive species |
| Full Name | mediator complex subunit 19 |
| Calculated Molecular Weight | 194 aa, 20 kDa |
| Observed Molecular Weight | 31 kDa |
| GenBank Accession Number | BC037223 |
| Gene Symbol | MED19 |
| Gene ID (NCBI) | 219541 |
| RRID | AB_10644134 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A0JLT2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mediator of RNA polymerase II transcription subunit 19 (MED19) is a component of the Mediator complex, which is a coactivator for DNA-binding factors that activate transcription via RNA polymerase II (PMID: 12584197). Mediator complex is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (PMID: 15175163). MED19 may be a novel proliferation regulator that promotes lung cancer and gastric cancer progression and cellular growth (PMID: 21519921, PMID: 22565189).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MED19 antibody 13418-1-AP | Download protocol |
| WB protocol for MED19 antibody 13418-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MED19 Polyclonal antibody (catalog #13418-1-AP) has 4 total citations. These appear in Molecular Cell, International Journal of Nanomedicine, Journal of Biochemical and Molecular Toxicology, and Journal of Cellular Biochemistry. The associated research fields span transcription regulation (RNA polymerase II pause release), nerve regeneration via exosomal circRNA/miRNA pathways, breast cancer progression and chemoresistance, and autophagy-mediated drug resistance. Applications cited include Western blot and immunohistochemistry in human and rat samples.
| Species | Application | Title |
|---|---|---|
Mol Cell The phosphatase PP1 sustains global transcription by promoting RNA polymerase II pause release | ||
Int J Nanomedicine Hypoxic Bone Mesenchymal Stem Cell-Derived Exosomes Direct Schwann Cells Proliferation, Migration, and Paracrine to Accelerate Facial Nerve Regeneration via circRNA_Nkd2/miR-214-3p/MED19 Axis | ||
J Biochem Mol Toxicol Chloroprocaine antagonizes progression of breast cancer by regulating LINC00494/miR-3619-5p/MED19 axis | ||
J Cell Biochem Med19 is involved in chemoresistance by mediating autophagy through HMGB1 in breast cancer.
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