Tested Applications
| Positive WB detected in | SH-SY5Y cells, A375 cells, U2OS cells |
| Positive IHC detected in | human stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27850-1-AP targets MED23 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27241 Product name: Recombinant human MED23 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 745-1059 aa of BC060759 Sequence: QNNVPQESRFNLKKNVEEEYRKWKSMSNENDIITHFSMQGSPPLFLCLLWKMLLETDHINQIGYRVLERIGARALVAHVRTFADFLVYEFSTSAGGQQLNKCIEILNDMVWKYNIVTLDRLILCLAMRSHEGNEAQVCYFIIQLLLLKPNDFRNRVSDFVKENSPEHWLQNDWHTKHMNYHKKYPEKLYFEGLAEQVDPPVQIQSPYLPIYFGNVCLRFLPVFDIVIHRFLELLPVSKSLETLLDHLGGLYKFHDRPVTYLYNTLHYYEMHLRDRAFLKRKLVHAIIGSLKDNRPQGWCLSDTYLKCAMNAREEN Predict reactive species |
| Full Name | mediator complex subunit 23 |
| Calculated Molecular Weight | 156 kDa |
| Observed Molecular Weight | 140 kDa, 50 kDa |
| GenBank Accession Number | BC060759 |
| Gene Symbol | MED23 |
| Gene ID (NCBI) | 9439 |
| RRID | AB_2880994 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULK4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED23 is a component of the Mediator complex, it is a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MED23 antibody 27850-1-AP | Download protocol |
| WB protocol for MED23 antibody 27850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MED23 polyclonal antibody (Cat# 27850-1-AP) has 1 total citation. It appears in Cell Reports (2021), where it was used in Western blot to study DDB1-mediated activation of the BRWD3 transcriptional cascade in adipogenesis and obesity onset in mouse models. The research field encompasses transcriptional regulation, adipocyte differentiation, and metabolic disease.
| Species | Application | Title |
|---|---|---|
Cell Rep DDB1 binds histone reader BRWD3 to activate the transcriptional cascade in adipogenesis and promote onset of obesity. |
Reviews
What customers say
The single review for this product was posted in April 2021 by a user performing Western Blot at a 1:500 dilution on embryo heart tissue. The reviewer rated it 2 out of 5 stars and noted that the antibody performed poorly, producing too many unspecific bands. No other reviews are available for this catalog number.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
SITING (Verified Customer) (04-02-2021) | not very good， too many unspecific band
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