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Tested Applications
| Positive WB detected in | Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17652-1-AP targets MED27 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MED27 fusion protein Ag11897 Predict reactive species |
| Full Name | mediator complex subunit 27 |
| Calculated Molecular Weight | 311 aa, 35 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC064608 |
| Gene Symbol | MED27 |
| Gene ID (NCBI) | 9442 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P2C8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED27 is a subunit of the Mediator complex, a large multiprotein complex that acts as a bridge between DNA-binding transcription factors and the RNA polymerase II transcriptional machinery. It is involved in the regulation of gene transcription and is essential for the initiation of transcription. The protein is highly conserved across different species, highlighting its fundamental role in cellular processes. It also influences cellular growth, development, and response to environmental cues.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED27 antibody 17652-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
