MED28 Polyclonal antibody

MED28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16262-1-AP

Synonyms

1500003D12Rik, EG1, Endothelial derived protein 1, magicin, MED28, mediator complex subunit 28, Tumor angiogenesis marker EG 1

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Validation Data Gallery

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  WB analysis using 16262-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 16262-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 16262-1-AP (MED28 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 16262-1-AP (MED28 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16262-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16262-1-AP (MED28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, PC-12 cells, rat testis tissue
• Positive IHC detected in: mouse testis tissue, mouse brain tissue, human kidney tissue, human placenta tissue, human testis tissue, human brain tissue, human liver tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16262-1-AP targets MED28 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MED28 fusion protein Ag9327
• Full Name: mediator complex subunit 28
• Calculated Molecular Weight: 178 aa, 20 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC011936
• Gene Symbol: MED28
• Gene ID (NCBI): 80306
• RRID: AB_2281863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.