MED6 Polyclonal antibody

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  WB analysis using 15338-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15338-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 15338-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15338-1-AP (MED6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 15338-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15338-1-AP (MED6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MED6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15338-1-AP

Publications(1)

Reviews (2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

ARC33, hMed6, MED6, mediator complex subunit 6, NY REN 28

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, HepG2 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15338-1-AP targets MED6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7541

  Product name: Recombinant human MED6 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-246 aa of BC004106

  Sequence: MAAVDIRDNLLGISWVDSSWIPILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYILLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFDEAMSYCRYHPSKGYWWHFKDHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKFPPKFVQLKPGEKPVPVDQTKKEAEPIPETVKPEEKETTKNVQQTVSAKGPPEKRMRLQ

• Full Name: mediator complex subunit 6
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC004106
• Gene Symbol: MED6
• Gene ID (NCBI): 10001
• RRID: AB_2142554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75586
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB	Mol Cell; An IDR-dependent mechanism for nuclear receptor control of Mediator interaction with RNA polymerase II; Authors - Haiyan Zhao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anh (Verified Customer) (04-18-2022)

claer band on target molecular weight , but sometimes have some unspecific band on membrane other size

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse tissue

FH

SITING (Verified Customer) (05-25-2021)

good

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED6 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:15338-1-AP)