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Tested Applications
| Positive WB detected in | A549 cells, Calu-3 cells, Daudi cells, MG-63 cells, PC-3 cells, T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
33458-1-AP targets MED7 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag38214 Product name: Recombinant human MED7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-233 aa of BC006968 Sequence: MGEPQQVSALPPPPMQYIKEYTDENIQEGLAPKPPPPIKDSYMMFGNQFQCDDLIIRPLESQGIERLHPMQFDHKKELRKLNMSILINFLDLLDILIRSPGSIKREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERVIEMIQNCLASLPDDLPHSEAGMRVKTEPMDADDSNNCTGQNEHQRENSGHRRDQIIEKDAALCVLIDEMNERP Predict reactive species |
| Full Name | mediator complex subunit 7 |
| Calculated Molecular Weight | 233 aa, 27 kDa |
| Observed Molecular Weight | 30 kDa |
| GenBank Accession Number | BC006968 |
| Gene Symbol | MED7 |
| Gene ID (NCBI) | 9443 |
| RRID | AB_3742979 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | O43513 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MED7 is a crucial subunit of the Mediator complex, a protein complex that regulates gene transcription by interacting with RNA polymerase II.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MED7 antibody 33458-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
