MEF2C Polyclonal antibody

MEF2C Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ChIP,ELISA

Conjugate

Unconjugated

Cat no : 18290-1-AP

Synonyms

MEF2C, myocyte enhancer factor 2C



Tested Applications

Positive WB detected inmouse cerebellum tissue, mouse brain tissue, mouse heart tissue, rat brain tissue
Positive IHC detected inhuman brain tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18290-1-AP targets MEF2C in WB, IHC, IF, ChIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name myocyte enhancer factor 2C
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa
GenBank accession numberNM_002397
Gene symbol MEF2C
Gene ID (NCBI) 4208
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. The immunogen used is peptide from Beta domain of MEF2C. The antibody can recognize the transcript variant 1 and transcript variant 3 of MEF2C.

Protocols

Product Specific Protocols
WB protocol for MEF2C antibody 18290-1-APDownload protocol
IHC protocol for MEF2C antibody 18290-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Biol Reprod

Integrated microRNA and mRNA network analysis of the human myometrial transcriptome in the transition from quiescence to labor.

Authors - William E Ackerman
mouseIF

Cell Rep

Chromatin Environment and Cellular Context Specify Compensatory Activity of Paralogous MEF2 Transcription Factors.

Authors - Shahriyar P Majidi
humanChIP

Nature

Evolution of Osteocrin as an activity-regulated factor in the primate brain.

Authors - Bulent Ataman