Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human placenta tissue, human heart tissue, human kidney tissue, human lung tissue, human ovary tissue, human skin tissue, human spleen tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18290-1-AP targets MEF2C in WB, IHC, IF-P, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | myocyte enhancer factor 2C |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 45-70 kDa |
| GenBank Accession Number | NM_002397 |
| Gene Symbol | MEF2C |
| Gene ID (NCBI) | 4208 |
| RRID | AB_2142849 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q06413 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEF2C belongs to the MEF2 family. It is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. MEF2C controls cardiac morphogenesis and myogenesis, and is also involved in vascular development[PMID: 20221419]. It plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission[PMID:18599438]. It is crucial for normal neuronal development, distribution, and electrical activity in the neocortex and is necessary for proper development of megakaryocytes and platelets and for bone marrow B lymphopoiesis[PMID: 21666133]. This protein is required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B cells. It may also be involved in neurogenesis and in the development of cortical architecture. MEF2C exists some isoforms with MV 50-52 kDa, 47 kDa, and 45 kDa, but modified MEF2C is about 55-66 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MEF2C antibody 18290-1-AP | Download protocol |
| IHC protocol for MEF2C antibody 18290-1-AP | Download protocol |
| WB protocol for MEF2C antibody 18290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MEF2C Polyclonal antibody (Cat# 18290-1-AP) has 8 citations published in Nature, Cell, Metabolism, Cell Genom, Cell Reports, International Journal of Molecular Sciences, and Biological Reproduction. Research fields span neuroscience (cortical interneuron development, parvalbumin interneuron maturation), epigenetics and chromatin biology, skeletal muscle mechanotransduction, cardiac development, Alzheimer's disease cis-regulatory elements, and reproductive biology (myometrial transcriptome). Applications cited include ChIP, WB, IHC, IF, and CoIP across human, mouse, and chicken species.
| Species | Application | Title |
|---|---|---|
Nature Genetic and epigenetic coordination of cortical interneuron development.
| ||
Cell A postnatal molecular switch drives activity-dependent maturation of parvalbumin interneurons. | ||
Metabolism PRR14 mediates mechanotransduction and regulates myofiber identity via MEF2C in skeletal muscle | ||
Cell Genom Single nucleus multiomics identifies ZEB1 and MAFB as candidate regulators of Alzheimer's disease-specific cis-regulatory elements | ||
Cell Rep Chromatin Environment and Cellular Context Specify Compensatory Activity of Paralogous MEF2 Transcription Factors. |
