Filter: AllWBIHC

WB analysis of mouse brain using 13456-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 13456-1-AP (MEI1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human cervical cancer using 13456-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 13456-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 13456-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 13456-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 13456-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 13456-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 13456-1-AP (MEI1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MEI1 Polyclonal antibody

Name: MEI1 Polyclonal antibody
Brand: Proteintech
SKU: 13456-1-AP

MEI1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13456-1-AP

Publications(1)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

MEI1, Meiosis defective protein 1, meiosis inhibitor 1, Meiosis inhibitor protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue
Positive IHC detected in	human cervical cancer tissue, human urothelial carcinoma tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13456-1-AP targets MEI1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4253 Product name: Recombinant human MEI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-238 aa of BC032248 Sequence: MLALTLAKADSPRTALLCSAWLLTASFSAQQHKGSLQVHQTLSVEMDQVLKALSFPKKKAALLSAAILCFLRTALRQSFSSALVALVPSGAQPLPATKDTVLAPLRMSQVRSLVIGLQNLLVQKDPLLSQACVGCLEALLDYLDARSPDIALHVASQPWNRFLLFTLLDAGENSFLRPEILRLMTLLQSMGHLADHSMAQTLQASLEGLPPSTSSGQPPLQDMLCLGGVAVSLSHIRN Predict reactive species
Full Name	meiosis inhibitor 1
Calculated Molecular Weight	894 aa, 99 kDa
Observed Molecular Weight	63 kDa
GenBank Accession Number	BC032248
Gene Symbol	MEI1
Gene ID (NCBI)	150365
RRID	AB_2877951
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5TIA1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEI1 may play a role in meiosis during spermatogenesis. It is required for normal meiotic chromosome synapsis. MEI1 may be involved in the formation of meiotic double-strand breaks (DSBs) in spermatocytes. This antibody recognizes isoform 1, 2, 3, 5, and 7 of MEI1 gene with MW 141 kDa, 67-72 kDa,49-54 kDa and 26 kDa .