MEIS2 Polyclonal antibody

MEIS2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 11550-1-AP

Synonyms

Homeobox protein Meis2, HsT18361, Meis homeobox 2, Meis1 related protein 1, MEIS2, MRG1

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Validation Data Gallery

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  WB analysis using 11550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11550-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11550-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11550-1-AP (MEIS2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11550-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11550-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11550-1-AP (MEIS2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, PC-3 cells, A549 cells, LNCaP cells, HCT 116 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

11550-1-AP targets MEIS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MEIS2 fusion protein Ag2096
• Full Name: Meis homeobox 2
• Calculated Molecular Weight: 381 aa, 42 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC001516
• Gene Symbol: MEIS2
• Gene ID (NCBI): 4212
• RRID: AB_2143028
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEIS2, also named as Meis1-related protein 1 or MRG1, is a 477 amino acid protein, which contains one homeobox DNA-binding domain and belongs to the TALE/MEIS homeobox family. MEIS2 is expressed in various tissues and at high level in the lymphoid organs of hematopoietic tissues. MEIS2 localizes in the nucleus and is involved in transcriptional regulation. MEIS2 is a critical downstream effector within an essential homeoprotein network that serving a rate-limiting regulatory role in MLL leukemogenesis. MEIS2 as midbrain specific marker is upregulated concomitantly with the morphological transformation of hindbrain to midbrain.

Publications

Species	Application	Title
mouse	IHC	Proc Natl Acad Sci U S A; Congenital diaphragmatic hernia candidate genes derived from embryonic transcriptomes.; Authors - Russell Meaghan K MK
mouse	WB	Aging Cell; Myeloid ectopic viral integration site 2 accelerates the progression of Alzheimer's disease; Authors - Yuting Cui ; KD Validated