Tested Applications
| Positive WB detected in | mouse liver tissue, A549 cells, rat liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12775-1-AP targets MEIS3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3471 Product name: Recombinant human MEIS3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 76-421 aa of BC025404 Sequence: CELATCSPRDGAGAGLGTPPGGDVCSSDSFNEDIAAFAKQVRSERPLFSSNPELDNLMIQAIQVLRFHLLELEKVHDLCDNFCHRYITCLKGKMPIDLVIEDRDGGCREDFEDYPASCPSLPDQNNMWIRDHEDSGSVHLGTPGPSSGGLASQSGDNSSDQGDGLDTSVASPSSGGEDEDLDQERRRNKKRGIFPKVATNIMRAWLFQHLSRRSEAPVLPDVCLGLGSPSPGPRWARPWGSDCGRPGRQSDSCWWLQHPYPSEEQKKQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRTGQGAAFSPEGQPIGGYTETQPHVAVRPPGSVGMSLNLEGEWHYL Predict reactive species |
| Full Name | Meis homeobox 3 |
| Calculated Molecular Weight | 421 aa, 46 kDa |
| Observed Molecular Weight | 46 kDa |
| GenBank Accession Number | BC025404 |
| Gene Symbol | MEIS3 |
| Gene ID (NCBI) | 56917 |
| RRID | AB_10639519 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99687 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MEIS3, also known as MRG2, belongs to the TALE/MEIS homeobox family. MEIS3 encodes a homeobox domain, so it can bind to DNA, regulate specific DNA sequence binding, and promote transcription activity. MEIS3 has 3 isoforms with the molecular mass of 39, 41, and 46 kDa (PMID: PMID: 33376716).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MEIS3 antibody 12775-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MEIS3 Polyclonal antibody (Cat# 12775-1-AP) has 1 publication citation. It appears in Molecular Systems Biology (IF 6.7), in a study investigating gene regulatory networks controlling the balance between mesendoderm and ectoderm at pluripotency exit. The research falls within the fields of developmental biology and stem cell biology, with the antibody used for Western blot in mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Syst Biol A gene regulatory network controls the balance between mesendoderm and ectoderm at pluripotency exit. |



