MEKK3 Polyclonal antibody

MEKK3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21072-1-AP

Synonyms

MAP3K3, MAPK/ERK kinase kinase 3, MAPKKK3, MEK kinase 3, MEKK 3, MEKK3



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 21072-1-AP is MEKK3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEKK3 fusion protein Ag15223
Full Name mitogen-activated protein kinase kinase kinase 3
Calculated molecular weight 657 aa, 74 kDa
Observed molecular weight 71 kDa, 74 kDa
GenBank accession numberBC090859
Gene symbol MAP3K3
Gene ID (NCBI) 4215
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mitogen-activated protein/ERK kinase kinase 3 (MEKK3) is a member of a family of MEKK/Ste11 serine/threonine protein kinases. One function of MEKK3 is to serve as an upstream regulatory kinase in the mitogen-activated protein kinase cascade, however, it also plays a critical role in other signaling pathways. MEKK3 regulates several important functions including cell survival and proliferation. It is ubiquitously expressed in multiple tissues and has been found in complexes with scaffold proteins and other kinases. (PMID: 18206350).Cerebral cavernous malformations (CCMs) complex regulation of MEKK3 is essential during vertebrate heart development. MEKK3 signalling and KLF2/4 may be targeted to develop new CCM therapeutics.

Protocols

Product Specific Protocols
WB protocol for MEKK3 antibody 21072-1-APDownload protocol
IHC protocol for MEKK3 antibody 21072-1-APDownload protocol
IP protocol for MEKK3 antibody 21072-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

Oncogene

MEKK2 and MEKK3 suppress Hedgehog pathway-dependent medulloblastoma by inhibiting GLI1 function.

Authors - Jinqiu Lu