MFI2 Recombinant monoclonal antibody, PBS Only (Capture)

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• MFI2 Recombinant monoclonal antibody, PBS Only (Capture) (88127-2-PBS)

MFI2 Uni-rAb^® Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 88127-2-PBS

Clone No.260392B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

Melanoma-associated antigen p97, Melanotransferrin, MELTF

• 

  WB analysis using 88127-2-RR (same clone as 88127-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 88127-2-RR (MELTF/CD228 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88127-2-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP03370-1

  The mean MFI2 concentration was determined to be 102.73 ng/mL in HEK-293 cell extract based on a 1.50 mg/mL extract load, 8.59 ng/mL in HeLa cell extract based on a 1.20 mg/mL extract load and 1.26 ng/mL in HL-60 cell extract based on a 1.20 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP03370-1

  Sandwich ELISA standard curve of MP03370-1, Human MFI2 Recombinant Matched Antibody Pair - PBS only. 88127-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg9197. 88127-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array sample test of MP03370-1

  The mean MFI2 concentration was determined to be 42.3 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load, 3.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load, 0.5 ng/mL in Raji cell extract based on a 1.5 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array standard curve of MP03370-1

  Cytometric bead array standard curve of MP03370-1, MFI2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88127-2-PBS. Detection antibody: 88127-3-PBS. Standard: Eg9197. Range: 0.312-40 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

88127-2-PBS targets MFI2 as part of a matched antibody pair:

MP03370-1: 88127-2-PBS capture and 88127-3-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg9197

  Product name: Recombinant human MELTF/CD228 protein

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 20-709 aa of NM_005929.6

  Sequence: GMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQC

• Full Name: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: NM_005929.6
• Gene Symbol: MFI2
• Gene ID (NCBI): 4241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08582-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological marker of colorectal cancer (PMID: 25216327).