MEP1A Polyclonal antibody

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• MEP1A Polyclonal antibody (24640-1-AP)

MEP1A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 24640-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PPHA, N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase subunit alpha, Meprin A subunit alpha, Endopeptidase-2, EC:3.4.24.18

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  WB analysis using 24640-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24640-1-AP (MEP1A antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse kidney using 24640-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24640-1-AP (MEP1A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 24640-1-AP

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 24640-1-AP (MEP1A antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24640-1-AP targets MEP1A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18950

  Product name: Recombinant human MEP1A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 365-713 aa of BC131714

  Sequence: DDSTGNVRKLVKVQTFQGDDDHNWKIAHVVLKEEQKFRYLFQGTKGDPQNSTGGIYLDDITLTETPCPTGVWTVRNFSQVLENTSKGDKLQSPRFYNSEGYGFGLTLYPNSGESSGYLRLAFHVCSGENDAILEWPVENRQVIITILDQEPDVRNRMSSSMVFTTSKSHTSPAINDTVIWDRPSRVGTYHTDCNCFRSIDLGWSGFISHQMLKRRSFLKNDDLIIFVDFEDITHLSQTEVPTKGKRLSPQGLILQGQEQQVSEEGSGKAMLEEALPVSLSQGQPSRQKRSVENTGPLEDHNWPQYFRDPCDPNPCQNDGICVNVKGMASCRCISGHAFFYTGERCQAVQ

• Full Name: meprin A, alpha (PABA peptide hydrolase)
• Calculated Molecular Weight: 746 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC131714
• Gene Symbol: MEP1A
• Gene ID (NCBI): 4224
• RRID: AB_2879650
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q16819
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The MEP1A Polyclonal antibody (Cat# 24640-1-AP) has 1 citation. It was used in an IHC application published in Computational and Structural Biotechnology Journal (2024), in a study on integrated immunogenomic analysis of single-cell and bulk profiling for identifying novel tumor antigens and subtype-specific therapeutic agents in lung adenocarcinoma. The research field is oncology, specifically lung cancer immunogenomics.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	IHC	Comput Struct Biotechnol J; Integrated immunogenomic analysis of single-cell and bulk profiling reveals novel tumor antigens and subtype-specific therapeutic agents in lung adenocarcinoma; Authors - Saisai Tian