MET Recombinant monoclonal antibody, PBS Only (Capture)

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• MET Recombinant monoclonal antibody, PBS Only (Capture) (82904-3-PBS)

MET Uni-rAb^® Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 82904-3-PBS

Clone No.253142A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

• 

  Sandwich ELISA sample test of MP03178-2

  The mean MET concentration was determined to be 46.22 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load, 20.18 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 30.19 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 1.80 ng/mL in SH-SY5Y cell extract based on a 1.5 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP03178-2

  Sandwich ELISA standard curve of MP03178-2, Human MET Recombinant Matched Antibody Pair - PBS only. 82904-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0230. 82904-5-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array sample test of MP03178-1

  The mean MET concentration was determined to be 40.2 ng/mL in A431cell extract based on a 1.5 mg/mL extract load, SH-SY5Y cell extract were served as a negative control.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Cytometric bead array standard curve of MP03178-1

  Cytometric bead array standard curve of MP03178-1, MET Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82904-3-PBS. Detection antibody: 82904-2-PBS. Standard:Eg0230. Range: 0.156-20 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Affinity of 82904-3-PBS

  Biolayer interferometry (BLI) kinetic assay of 82904-3-PBS against Human MET was performed. The affinity constant is 0.474 nM.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

82904-3-PBS targets MET as part of a matched antibody pair:

MP03178-1: 82904-3-PBS capture and 82904-2-PBS detection (validated in Cytometric bead array)

MP03178-2: 82904-3-PBS capture and 82904-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg0230

  Product name: Recombinant human MET protein

  Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

  Tag: C-6*HIS

  Domain: 25-932 aa of BC130420

  Sequence: ECKEALAKSEMNVNMKYQLPNFTAETPIQNVILHEHHIFLGATNYIYVLNEEDLQKVAEYKTGPVLEHPDCFPCQDCSSKANLSGGVWKDNINMALVVDTYYDDQLISCGSVNRGTCQRHVFPHNHTADIQSEVHCIFSPQIEEPSQCPDCVVSALGAKVLSSVKDRFINFFVGNTINSSYFPDHPLHSISVRRLKETKDGFMFLTDQSYIDVLPEFRDSYPIKYVHAFESNNFIYFLTVQRETLDAQTFHTRIIRFCSINSGLHSYMEMPLECILTEKRKKRSTKKEVFNILQAAYVSKPGAQLARQIGASLNDDILFGVFAQSKPDSAEPMDRSAMCAFPIKYVNDFFNKIVNKNNVRCLQHFYGPNHEHCFNRTLLRNSSGCEARRDEYRTEFTTALQRVDLFMGQFSEVLLTSISTFIKGDLTIANLGTSEGRFMQVVVSRSGPSTPHVNFLLDSHPVSPEVIVEHTLNQNGYTLVITGKKITKIPLNGLGCRHFQSCSQCLSAPPFVQCGWCHDKCVRSEECLSGTWTQQICLPAIYKVFPNSAPLEGGTRLTICGWDFGFRRNNKFDLKKTRVLLGNESCTLTLSESTMNTLKCTVGPAMNKHFNMSIIISNGHGTTQYSTFSYVDPVITSISPKYGPMAGGTLLTLTGNYLNSGNSRHISIGGKTCTLKSVSNSILECYTPAQTISTEFAVKLKIDLANRETSIFSYREDPIVYEIHPTKSFISGGSTITGVGKNLNSVSVPRMVINVHEAGRNFTVACQHRSNSEIICCTTPSLQQLNLQLPLKTKAFFMLDGILSKYFDLIYVHNPVFKPFEKPVMISMGNENVLEIKGNDIDPEAVKGEVLKVGNKSCENIHLHSEAVLCTVPNDLLKLNSELNIEWKQAISSTVLGKVIVQPDQNFT

• Full Name: met proto-oncogene (hepatocyte growth factor receptor)
• Calculated Molecular Weight: 1390 aa, 155 kDa
• GenBank Accession Number: BC130420
• Gene Symbol: MET
• Gene ID (NCBI): 4233
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08581
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.