METRN Polyclonal antibody

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  WB analysis using 16327-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16327-1-AP (METRN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 16327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 16327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 16327-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 16327-1-AP (METRN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

METRN Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16327-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C16orf23, Meteorin, RJD6

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16327-1-AP targets METRN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9414

  Product name: Recombinant human METRN protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 25-293 aa of BC000662

  Sequence: YSEERCSWRGSGLTQEPGSVGQLALACAEGAVEWLYPAGALRLTLGGPDPRARPGIACLRPVRPFAGAQVFAERAGGALELLLAEGPGPAGGRCVRWGPRERRALFLQATPHQDISRRVAAFRFELREDGRPELPPQAHGLGVDGACRPCSDAELLLAACTSDFVIHGIIHGVTHDVELQESVITVVAARVLRQTPPLFQAGRSGDQGLTSIRTPLRCGVHPGPGTFLFMGWSRFGEARLGCAPRFQEFRRAYEAARAAHLHPCEVALH

• Full Name: meteorin, glial cell differentiation regulator
• Calculated Molecular Weight: 31 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC000662
• Gene Symbol: METRN
• Gene ID (NCBI): 79006
• RRID: AB_3669238
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJH8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Meteorin, also named as METRN and C16orf23, is a secret protein which plays important roles in both glial cell differentiation and axonal network formation during neurogenesis.