METTL17 Polyclonal antibody

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• METTL17 Polyclonal antibody (20890-1-AP)

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  IHC staining of mouse colon using 20890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20890-1-AP (METT11D1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 20890-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20890-1-AP (METT11D1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

METTL17 Polyclonal Antibody for IHC, ELISA

Cat No. 20890-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF, ELISA

Synonyms

METT11D1, False p73 target gene protein, Methyltransferase 11 domain-containing protein 1, Methyltransferase-like protein 17, Ribosome assembly protein METTL17, mitochondrial

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Tested Applications

• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

20890-1-AP targets METTL17 in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14518

  Product name: Recombinant human METT11D1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC005053

  Sequence: MAAALKCLLTLGRWCPGLGVAPQARALAALVPGVTQVDNKSGFLQKRPHRQHPGILKLPHVRLPQALANGAQLLLLGSAGPTMENQVQTLTSYLWSRHLPVEPEELQRRARHLEKKFLENPDLSQTEEKLRGAVLHALRKTTYHWQELSYTEGLSLVYMAARLDGGFAAVSRAFHEIRARNPAFQPQTLMDFGSGTGSVTWAAHSIWGQSLREYMCVDRSAAMLVLAEKLLKGGSESGEPYIPGVFFRQFLPVSPKVQFDVVVSAFSLSELPSKADRTEVVQTLWRKTGHFLVLVENGTKAGHSLLMDARDLVLKGKEKSPLDPRPGFVFAPCPHELPCPQLTNLACSFSQ

• Full Name: methyltransferase 11 domain containing 1
• Calculated Molecular Weight: 478 aa, 53 kDa
• GenBank Accession Number: BC005053
• Gene Symbol: METT11D1
• Gene ID (NCBI): 64745
• RRID: AB_3742007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H7H0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

METTL17 is located in mitochondria through its N-terminal targeting sequence and specifically interacts with 12S mitochondrial ribosomal RNA (mt-rRNA) as well as small subunits of mitochondrial ribosome (MSSUs). METTL17, a regulator of mitochondrial ribosomal RNA modifications, is required for the translation of mitochondrial coding genes. (PMID: 38377789, PMID: 31487196)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IF	Eur J Med Chem; Bufalin targeting METTL17 inhibits the occurrence and metastasis of oral cancer through JAK1/STAT3 signaling pathway.; Authors - Huhu Zhang