METTL3 Monoclonal antibody

METTL3 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

4B4A3

Cat no : 67733-2-Ig

Synonyms

IME4, M6A, methyltransferase like 3, METTL3, MT A70, MTA70, Spo8



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human urothelial carcinoma tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Sample-dependent, check data in validation data gallery

Product Information

67733-2-Ig targets METTL3 in WB, IHC,ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen METTL3 fusion protein Ag7110
Full Name methyltransferase like 3
Calculated molecular weight 64 kDa
Observed molecular weight70 kDa
GenBank accession numberBC001650
Gene symbol METTL3
Gene ID (NCBI) 56339
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

METTL3 is a key S-adenosyl-L-methionine-binding subunit, which is component of a complex multicomponent enzyme that catalyzes the methylation of internal adenosine residues in eukaryotic mRNA, forming N6-methyladenosine. It contains 2 putative nuclear localization signals and 2 consensus methylation motifs. The calculated molecular weight of METTL3 is 64 kDa, but modified METTL3 is about 65-70 kDa.

Protocols

Product Specific Protocols
WB protocol for METTL3 antibody 67733-2-IgDownload protocol
IHC protocol for METTL3 antibody 67733-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols