MFAP4 Polyclonal antibody

MFAP4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 17661-1-AP

Synonyms

MFAP4

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Validation Data Gallery

• 

  WB analysis of human kidney using 17661-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat lung using 17661-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

• 

  WB analysis of mouse heart using 17661-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 17661-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17661-1-AP (MFAP4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17661-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17661-1-AP (MFAP4 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human kidney tissue, mouse heart tissue, mouse lung tissue, rat lung tissue
• Positive IHC detected in: human ovary tissue, human lung tissue, human placenta tissue, human skin tissue, human spleen tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

17661-1-AP targets MFAP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFAP4 fusion protein Ag11920
• Full Name: microfibrillar-associated protein 4
• Calculated Molecular Weight: 255 aa, 29 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC062415
• Gene Symbol: MFAP4
• Gene ID (NCBI): 4239
• RRID: AB_2142355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)

Publications

Species	Application	Title
human	WB, IHC	Thorax; A large lung gene expression study identifying fibulin-5 as a novel player in tissue repair in COPD.; Authors - Corry-Anke Brandsma
human	WB	J Proteome Res; Strategy for SRM-based verification of biomarker candidates discovered by iTRAQ method in limited breast cancer tissue samples.; Authors - Muraoka Satoshi S
human	IHC, IF	FASEB J; The "Matrisome" reveals the characterization of skin keloid microenvironment.; Authors - Shikun Zhang
mouse	WB	J Proteomics; FOXL1 + Telocytes in mouse colon orchestrate extracellular matrix biodynamics and wound repair resolution; Authors - Veronique Pomerleau
mouse	WB,IF	BMC Biol; The m6A writer KIAA1429 regulates photoaging progression via MFAP4-dependent collagen synthesis; Authors - Yuanyuan Liu