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MFAP4 Recombinant monoclonal antibody (88535-1-RR)

MFAP4 Recombinant monoclonal antibody

MFAP4 Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 88535-1-RR

Clone No.261177D10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Microfibril-associated glycoprotein 4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 88535-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88535-1-RR (MFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 88535-1-RR
Various lysates were subjected to SDS PAGE followed by western blot with 88535-1-RR (MFAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 88535-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 88535-1-RR
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 88535-1-RR
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human lung using 88535-1-RR
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 88535-1-RR (MFAP4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse lung tissue, mouse spleen tissue, rat lung tissue, rat spleen tissue
Positive IHC detected in	human colon tissue, human lung tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

88535-1-RR targets MFAP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag11920 Product name: Recombinant human MFAP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-255 aa of BC062415 Sequence: MKALLALPLLLLLSTPPCAPQVSGIRGDALERFCLQQPLDCDDIYAQGYQSDGVYLIYPSGPSVPVPVFCDMTTEGGKWTVFQKRFNGSVSFFRGWNDYKLGFGRADGEYWLGLQNMHLLTLKQKYELRVDLEDFENNTAYAKYADFSISPNAVSAEEDGYTLFVAGFEDGGVGDSLSYHSGQKFSTFDRDQDLFVQNCAALSSGAFWFRSCHFANLNGFYLGGSHLSYANGINWAQWKGFYYSLKRTEMKIRRA Predict reactive species
Full Name	microfibrillar-associated protein 4
Calculated Molecular Weight	255 aa, 29 kDa
Observed Molecular Weight	36 kDa
GenBank Accession Number	BC062415
Gene Symbol	MFAP4
Gene ID (NCBI)	4239
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P55083
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microfibrillar-associated protein 4 (MFAP4) is a extracellular matrix glycoprotein containing a fibrinogen C-terminal domain and a N-terminal located integrin-binding motif. It could be involved in calcium-dependent cell adhesion or intercellular interactions. MFAP4 gene is commonly deleted in Smith-Magenis syndrome patients. (PMID: 7633408; 16867155; 10542261; 18322703)