MFI2 Polyclonal antibody

MFI2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10428-1-AP

Synonyms

MELTF, Melanotransferrin, Melanoma-associated antigen p97

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Validation Data Gallery

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  WB analysis of HEK-293 using 10428-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10428-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 10428-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 10428-1-AP (MFI2 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary cancer using 10428-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 10428-1-AP (MFI2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Transfected
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

10428-1-AP targets MFI2 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MFI2 fusion protein Ag0700
• Full Name: antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5
• Calculated Molecular Weight: 80 kDa
• Observed Molecular Weight: 82 kDa
• GenBank Accession Number: BC001875
• Gene Symbol: MFI2
• Gene ID (NCBI): 4241
• RRID: AB_2142592
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MFI2 (Melanotransferrin/Mtf/TRMF), also known as p97 or CD228, is an iron-binding glycoprotein belonging to the transferrin family. Alternative RNA splicing yields two forms of different length transcripts, one (the longer) bound to the membrane (mMFI2) and the other (the shorter) secreted to the peripheral environment (sMFI2) such as blood and cell supernatant. MFI2 is upregulated in tumor tissues and has been identified as a serological marker of colorectal cancer (PMID: 25216327).

Publications

Species	Application	Title
mouse	WB	Cell Death Discov; Gsα deficiency facilitates cardiac remodeling via CREB/ Bmp10-mediated signaling; Authors - Ping Yin
human,rat	WB,IF	Reprod Sci; MELTF Might Regulate Ferroptosis, Pyroptosis, and Autophagy in Platelet-Rich Plasma-Mediated Endometrial Epithelium Regeneration; Authors - Yanhong Mao
human	WB	Front Immunol; Identification of novel gene signature for lung adenocarcinoma by machine learning to predict immunotherapy and prognosis; Authors - Jianfeng Shu