Tested Applications
| Positive WB detected in | HSC-T6 cells, T-47D cells, mouse brain tissue, mouse kidney tissue, mouse liver tissue |
| Positive IP detected in | mouse kidney tissue |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13798-1-AP targets MFN1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, monkey, chicken, hamster, goat, ducks |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4762 Product name: Recombinant human MFN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-351 aa of BC040557 Sequence: EFVTEGSHFVEATYKNPELDRIATEDDLVEMQGYKDKLSIIGEVLSRRHMKVAFFGRTSSGKSSVINAMLWDKVLPSGIGHITNCFLSVEGTDGDKAYLMTEGSDEKKSVKTVNQLAHALHMDKDLKAGCLVRVFWPKAKCALLRDDLVLVDSPGTDVTTELDSWIDKFCLDADVFVLVANSESTLMNTEKHFFHKVNERLSKPNIFILNNRWDASASEPEYMEDVRRQHMERCLHFLVEELKVVNALEAQNRIFFVSAKEVLSARKQKAQGMPESGVALAEGFHARLQEFQNFEQIFEECISQSAVKTKFEQHTIRAKQILATV Predict reactive species |
| Full Name | mitofusin 1 |
| Calculated Molecular Weight | 741 aa, 84 kDa |
| Observed Molecular Weight | 84 kDa |
| GenBank Accession Number | BC040557 |
| Gene Symbol | MFN1 |
| Gene ID (NCBI) | 55669 |
| RRID | AB_2266318 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWA4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mitofusin-1 (MFN1) is a mediator of mitochondrial fusion. This protein and mitofusin 2 are homologs of the Drosophila protein fuzzy onion (Fzo). Mitofusins are large predicted GTPases located in the outer mitochondrial membrane. They are essential for outer membrane fusion by interacting with each other to facilitate mitochondrial targeting. The mitofusins are the first known protein mediator of mitochondrial fusion, and mediate developmentally regulated post-meiotic fusion of mitochondria.Mfn1 is required on adjacent mitochondria to mediate fusion via interactions of a heptad repeat region that mediates oligomerization of the protein(PMID:16892085). Mitofusin 1 and mitofusin 2 are ubiquitinated in a PINK1/parkin-dependent manner upon induction of mitophagy(PMID: 20871098).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MFN1 antibody 13798-1-AP | Download protocol |
| IHC protocol for MFN1 antibody 13798-1-AP | Download protocol |
| IP protocol for MFN1 antibody 13798-1-AP | Download protocol |
| WB protocol for MFN1 antibody 13798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MFN1 Polyclonal antibody (catalog no. 13798-1-AP) has 497 total citations. These appear in high-impact journals including Nature, Science, Cell, Cell Metabolism, Nature Communications, Cell Death & Differentiation, Autophagy, Redox Biology, Free Radical Biology and Medicine, and Journal of Clinical Investigation. Research fields span mitochondrial dynamics (fusion/fission), mitophagy, and mitochondrial quality control, with applications in cancer, neurodegeneration (Parkinson's, Alzheimer's), cardiovascular disease, diabetes, fibrosis, and metabolic disorders.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma. | ||
Nature Cellular ATP demand creates metabolically distinct subpopulations of mitochondria
| ||
Science Endosomal lipid signaling reshapes the endoplasmic reticulum to control mitochondrial function | ||
Cell Mitocytosis, a migrasome-mediated mitochondrial quality-control process.
| ||
Cell Metab Astrocytic LRP1 enables mitochondria transfer to neurons and mitigates brain ischemic stroke by suppressing ARF1 lactylation |
Reviews
What customers say
Customers rate this antibody highly, with most reviews at 4–5 stars. It performs well in Western Blot across various cell lines (HeLa, T cells, HCEC, ARPE-19, A549, corneal cells) and tissues, with good resolution and reliable band detection. Several users also confirmed successful use in IF, IHC, IP, and flow cytometry. One reviewer noted occasional non-specific binding, similar to other MFN2 antibodies. A single lower-rated review reported it did not work on rat liver tissue. Overall, users describe it as a cost-effective, dependable reagent with consistent performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chun (Verified Customer) (12-22-2025) | This antibody works well in Immunoblotting.
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Pierre (Verified Customer) (09-26-2025) | Very good
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Dhanwini (Verified Customer) (09-24-2025) | Good
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Robert (Verified Customer) (09-24-2025) | Similar to MFN2, gets the job done, but still expect some non-specific binding
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Sneha (Verified Customer) (09-24-2025) | GREAT
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Michael (Verified Customer) (09-23-2025) | Works very well with western!
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Henry (Verified Customer) (09-23-2025) | Good resolution
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S (Verified Customer) (09-23-2024) | Good
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Ruiting (Verified Customer) (09-18-2020) | Always purchase antibodies from your company, antibody is cheap, and works well. Never has a bad experience in any antibody. Plan to buy two more next week.
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Kishor (Verified Customer) (01-30-2019) | I working good for Western Blotting (1:1000) in human cancer cells but not for rat liver tissue
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